Aop:201

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Status

This is a legacy representation of this AOP. Please see the current version here:

https://aopwiki.org/aops/201

AOP Title

Juvenile hormone receptor agonism leading to male offspring induction associated population decline
Short name: JHR agonism leading to population decline

Authors

You Song1 and Knut Erik Tollefsen1,2

1 Norwegian Institute for Water Research (NIVA), Section of Ecotoxicology and Risk Assessment, Gaustadalléen 21, N-0349 Oslo, Norway

2 Norwegian University of Life Sciences (NMBU), Faculty of Environmental Science and Technology, Department of Environmental Sciences (IMV). P.O. Box 5003, N-1432 Ås, Norway

Contact: knut.erik.tollefsen@niva.no

Abstract

Endocrine disruption (ED) associated with environmental sex determination (ESD) and reproduction effects has been frequently reported in various ecotoxicologcail studies with crustaceans, such as daphniids (reviewed in (LeBlanc and Medlock, 2015)). Daphniids normally go through parthenogenic reproduction cycles in which only female offspring are produced. When under environmental stress (e.g. chemical exposure, short length of daylight, food shortage and high population density), a switch of reproductive strategy from asexual to sexual reproduction occurs and male offspring are produced. As an indicator of ED, the induction of male offspring is used as an important endpoint in daphnia toxicity tests. It has been widely recognized that analogs of the crustacean juvenile hormone (JH), methyl farnesoate (MF) are able to bind and activate the crustacean JH receptor, methoprene tolerant (Met), thus inducing the putative sex determination genes, doublesex genes, and causing male offspring formation. The induction of male neonates is usually associated with reduced fecundity and population decline as adverse ED effects. The existing evidences from different studies currently support the causal relationships between the biological events occurred in daphniids after exposure to JH analogs and subsequently form an adverse outcome pathway (AOP) as proposed herein.


Background (optional)

Summary of the AOP

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Molecular Initiating Event

Molecular Initiating Event Support for Essentiality
Juvenile hormone receptor, Activation Strong

Key Events

Event Support for Essentiality
Doublesex1 gene, Induction
Male reproductive tract, Induction

Adverse Outcome

Adverse Outcome
Male offspring, Increased
Population, Decline
Food-web structures, Alteration

Relationships Among Key Events and the Adverse Outcome

Event Description Triggers Weight of Evidence Quantitative Understanding
Juvenile hormone receptor, Activation Directly Leads to Doublesex1 gene, Induction
Doublesex1 gene, Induction Directly Leads to Male reproductive tract, Induction
Male reproductive tract, Induction Directly Leads to Male offspring, Increased
Male offspring, Increased Directly Leads to Population, Decline
Population, Decline Indirectly Leads to Food-web structures, Alteration

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Life Stage Applicability

Life Stage Evidence Links
Adults

Taxonomic Applicability

Name Scientific Name Evidence Links
Daphnia magna Daphnia magna NCBI
Daphnia pulex Daphnia pulex NCBI

Sex Applicability

Sex Evidence Links
Female

Graphical Representation

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Overall Assessment of the AOP

Domain of Applicability

Life Stage Applicability This AOP applies to: juvenile (<24h old) exposed to the MF analogs during a chronic (>14d) exposure (Abe et al., 2014a); and sex-mature adult (>10d old) exposed to MF analogs in the middle of a reproduction cycle for at least three days (Abe et al., 2014a).

Taxonomic Applicability This AOP can be potentially applied to other crustacean species and insects. The ligand binding domain of Met is considered to be conserved across different daphnia species and insects (Miyakawa et al., 2013). The juvenile hormone III (methyl farnesoate) signaling pathway to exert hormonal actions are thought to be similar between daphnids and insects with only two amino acids being different, which enhances the responsiveness of the Met receptor to various juvenile hormone III analogs (Miyakawa et al., 2013).

Sex Applicability
This AOP applies to females.

Elaborate on the domains of applicability listed in the summary section above. Specifically, provide the literature supporting, or excluding, certain domains.

Essentiality of the Key Events

Molecular Initiating Event Summary

The support for essentiality for MiE is considered to be strong. It has been shown that MF and the MF analog pyriproxyfen activated the cloned Daphnia pulex Met when expressed with the mosquito SRC ortholog (LeBlanc et al., 2013). A two-hybrid in vitro screening bioassay has been developed using transfected Chinese hamster ovary (CHO) cells containing the transcriptional activity of Met/SRC for screening of Met activation by MF analogs (Miyakawa et al., 2013). The application of this screening assay by different studies supported that chemical with MF activities, including MF, juvenile hormone III, fenoxycarb (Miyakawa et al., 2013) and diofenolan (Abe et al., 2015a) were able to activate the Met receptor and initiate downstream transcriptional regulation in a concentration-dependent manner. Evidences generated from this screening assay quantitatively demonstrated the causal relationship between chemical exposure and transcriptional activation of Met.

Key Event Summary
The dsx1 gene has shown to be highly responsible for the regulation of male traits development in the embryos of D. magna after exposure to the MF analog fenoxycarb (Kato et al., 2011). The same study has also shown that lack of dsx1 gene expression in male embryos of D. magna resulted in all female phenotypes. A high number of studies have quantitatively assessed the effects of MF-like chemicals on the induction of male offspring in daphnids (Abe et al., 2015b; Abe et al., 2014b; Ginjupalli and Baldwin, 2013; Kim et al., 2011; Lampert et al., 2012; Matsumoto et al., 2008; Oda et al., 2007; Oda et al., 2005; Olmstead and LeBlanc, 2001a, 2003; Olmstead and LeBlanc, 2001b; Tatarazako et al., 2003; Wang et al., 2005). These studies strongly support the induction of male offspring as a results of MF-related ED effects. Therefore, it is evident that the support for essentiality of Met receptor activation by MF analogs leading to the induction of dsx1, male traits and male offspring is strong.

Provide an overall assessment of the essentiality for the key events in the AOP. Support calls for individual key events can be included in the molecular initiating event, key event, and adverse outcome tables above.

Weight of Evidence Summary

Summary Table
The overall weight of evidence (WoE) for this AOP is considered to be moderate. The WoE for each individual KER is summarized in the overview table above. The MiE of Met receptor activation leading to the induction of dsx1 gene is considered to be strong. It has been demonstrated in insects such as Drosophila that the direct target gene following the MF receptor activation was the sex lethal gene (sxl) (Zhang et al., 2014). The sxl protein as an RNA splicing factor then leads to the expression of the transformer (TRA) gene and protein to regulate the doublesex (dsx) genes (Kopp, 2012). However, studies have shown that the tra gene was not involved in the sex determination in daphnids (Kato et al., 2010). Therefore, intermediate targets between the Met receptor activation and the induction of dsx genes still need to be found. Nevertheless, the dsx1 gene has shown to be highly responsible for the regulation of male traits development in the embryos of D. magna after exposure to the MF analog fenoxycarb (Kato et al., 2011). The same study has also shown that lack of dsx1 gene expression in male embryos of D. magna resulted in all female phenotypes. Therefore, it is evident that the support for essentiality of Met receptor activation by MF analogs leading to the induction of dsx1 and male traits formation is strong. The induction of dsx1 genes and male traits development leading to the induction of male offspring is considered to be strong. A study by (Miyakawa et al., 2013) reported good correlation between the EC50s of male offspring induction and EC50s of Met receptor activation for MF-like chemicals, including MF, fenoxycarb, JH III and pyriproxyfen. The induction of male offspring leading to reduction of fecundity is considered to be moderate. A number of studies have shown that the reduction of fecundity often occurred with the increased male:female ratio of offspring in daphnia after exposure to MF analogs (Abe et al., 2015b; Abe et al., 2014b; Ginjupalli and Baldwin, 2013; Kim et al., 2011; Lampert et al., 2012; Matsumoto et al., 2008; Oda et al., 2007; Oda et al., 2005; Olmstead and LeBlanc, 2001a, 2003; Olmstead and LeBlanc, 2001b; Tatarazako et al., 2003; Wang et al., 2005). Although the underlying mechanism and quantitative causal relationship between male formation and reduced reproduction has not been well-characterized, these evidences support the hypothesis that reduction of fecundity may be caused by disrupted sex determination pathway. The reduction of fecundity and induction of male offspring leading to the decline of population size is considered to be weak. Although lack of direct experimental evidences, it may be deduced that the most efficient way of reproduction, parthenogenesis is disrupted, thus the population size in the next generations may decrease. The decline of population size leading to the disturbance of food-web structure is considered to be weak. Although lack of experimental evidences, it may deduced that the food-web structure may be disrupted due to the decline of daphnia population.

Provide an overall summary of the weight of evidence based on the evaluations of the individual linkages from the Key Event Relationship pages.

Quantitative Considerations

Summary Table
The mechanistic evidences between the MiE of Met activation and induction of dsx1 gene have been clearly shown by various studies. However, the concentration-response relationship still needs to be obtained. A combination of the two-hybrid screening assay and quantitative real-time reverse transcriptase polymerase chain reaction (qPCR) would greatly facilitate the quantification of this KER. So this KER is considered to be moderate in terms of quantitative considerations. Demonstrations of the KER between the induction of dsx1 gene and male traits formation in the embryos using a combination of qPCR and in situ hybridization have been clearly provided (Kato et al., 2010). However, concentration-response relationship for this KER has not been well determined. So this KER is considered to be moderate in terms of quantitative considerations. The mechanism underlying the induction of male offspring leading to fecundity reduction has not been well characterized, and not quantitative assessment has been performed. So this KER is considered to be weak in terms of quantitative considerations. Linkages between the fecundity reduction, population decline and food-web disturbance have not been well studied. So these KERs are considered to be weak in terms of quantitative considerations.

Provide an overall discussion of the quantitative information available for this AOP. Support calls for the individual relationships can be included in the Key Event Relationship table above.

Considerations for Potential Applications of the AOP (optional)

References

Abe, R., Toyota, K., Miyakawa, H., Watanabe, H., Oka, T., Miyagawa, S., Nishide, H., Uchiyama, I., Tollefsen, K.E., Iguchi, T., Tatarazako, N., 2014. Diofenolan induces male offspring production through binding to the juvenile hormone receptor in Daphnia magna. Aquatic toxicology 159C, 44-51.

Abe, R., Watanabe, H., Yamamuro, M., Iguchi, T., Tatarazako, N., 2014b. Establishment of a short-term, in vivo screening method for detecting chemicals with juvenile hormone activity using adult Daphnia magna. Journal of applied toxicology : JAT.

Ginjupalli, G.K., Baldwin, W.S., 2013. The time- and age-dependent effects of the juvenile hormone analog pesticide, pyriproxyfen on Daphnia magna reproduction. Chemosphere 92, 1260-1266. Kato, Y., Kobayashi, K., Oda, S., Tatarazako, N., Watanabe, H., Iguchi, T., 2010. Sequence divergence and expression of a transformer gene in the branchiopod crustacean, Daphnia magna. Genomics 95, 160-165.

Kato, Y., Kobayashi, K., Watanabe, H., Iguchi, T., 2011. Environmental sex determination in the branchiopod crustacean Daphnia magna: deep conservation of a Doublesex gene in the sex-determining pathway. PLoS genetics 7, e1001345.

Kim, J., Kim, Y., Lee, S., Kwak, K., Chung, W.J., Choi, K., 2011. Determination of mRNA expression of DMRT93B, vitellogenin, and cuticle 12 in Daphnia magna and their biomarker potential for endocrine disruption. Ecotoxicology 20, 1741-1748.

Kopp, A., 2012. Dmrt genes in the development and evolution of sexual dimorphism. Trends Genet 28, 175-184.

Lampert, W., Lampert, K.P., Larsson, P., 2012. Induction of male production in clones of Daphnia pulex by the juvenoid hormone methyl farnesoate under short photoperiod. Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 156, 130-133.

LeBlanc, G.A., Medlock, E.K., 2015. Males on demand: the environmental-neuro-endocrine control of male sex determination in daphnids. FEBS J 282, 4080-4093.

LeBlanc, G.A., Wang, Y.H., Holmes, C.N., Kwon, G., Medlock, E.K., 2013. A transgenerational endocrine signaling pathway in Crustacea. PloS one 8, e61715.

Matsumoto, T., Ikuno, E., Itoi, S., Sugita, H., 2008. Chemical sensitivity of the male daphnid, Daphnia magna, induced by exposure to juvenile hormone and its analogs. Chemosphere 72, 451-456.

Miyakawa, H., Toyota, K., Hirakawa, I., Ogino, Y., Miyagawa, S., Oda, S., Tatarazako, N., Miura, T., Colbourne, J.K., Iguchi, T., 2013. A mutation in the receptor Methoprene-tolerant alters juvenile hormone response in insects and crustaceans. Nature communications 4, 1856.

Oda, S., Tatarazako, N., Dorgerloh, M., Johnson, R.D., Ole Kusk, K., Leverett, D., Marchini, S., Nakari, T., Williams, T., Iguchi, T., 2007. Strain difference in sensitivity to 3,4-dichloroaniline and insect growth regulator, fenoxycarb, in Daphnia magna. Ecotoxicology and environmental safety 67, 399-405.

Oda, S., Tatarazako, N., Watanabe, H., Morita, M., Iguchi, T., 2005. Production of male neonates in Daphnia magna (Cladocera, Crustacea) exposed to juvenile hormones and their analogs. Chemosphere 61, 1168-1174.

Olmstead, A.W., LeBlanc, G.A., 2001a. Temporal and quantitative changes in sexual reproductive cycling of the cladoceran Daphnia magna by a juvenile hormone analog. The Journal of experimental zoology 290, 148-155.

Olmstead, A.W., LeBlanc, G.A., 2003. Insecticidal juvenile hormone analogs stimulate the production of male offspring in the crustacean Daphnia magna. Environmental health perspectives 111, 919-924.

Olmstead, A.W., LeBlanc, G.L., 2001b. Low exposure concentration effects of methoprene on endocrine-regulated processes in the crustacean Daphnia magna. Toxicological sciences : an official journal of the Society of Toxicology 62, 268-273.

Tatarazako, N., Oda, S., Watanabe, H., Morita, M., Iguchi, T., 2003. Juvenile hormone agonists affect the occurrence of male Daphnia. Chemosphere 53, 827-833.

Wang, H.Y., Olmstead, A.W., Li, H., Leblanc, G.A., 2005. The screening of chemicals for juvenoid-related endocrine activity using the water flea Daphnia magna. Aquatic toxicology 74, 193-204.

Zhang, Z., Klein, J., Nei, M., 2014. Evolution of the sex-lethal gene in insects and origin of the sex-determination system in Drosophila. Journal of molecular evolution 78, 50-65.