Difference between revisions of "Event:920"

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(How this Key Event works)
(How it is Measured or Detected)
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4. Is the assay reproducible?
 
4. Is the assay reproducible?
 
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'''In vitro/in vivo experiments'''
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Increased mucus is detected using Alcian blue/periodic acid-Schiff (AB/PAS) for detection of mucin glycoproteins. In cell and animal experiments, literature equates an increase in mucus production with "mucus metaplasia" even though the study did not look at the origin of cells to see if they were differentiated from non-goblet cells.
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'''Clinical'''
  
 
== Evidence Supporting Taxonomic Applicability ==
 
== Evidence Supporting Taxonomic Applicability ==

Revision as of 00:13, 22 April 2016


Event Title

Metaplasia of goblet cells, Increase

Key Event Overview

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AOPs Including This Key Event

AOP Name Event Type Essentiality
EGFR Activation Leading to Mucus Hypersecretion KE Moderate

Taxonomic Applicability

Name Scientific Name Evidence Links

Level of Biological Organization

Biological Organization

How this Key Event works

Metaplasia is the replacement of a differentiated cell type with another differentiated cell type. In the case of goblet cell metaplasia, ciliated or Clara cells transdifferentiate into goblet cells (Tyner et al., 2006), (Evans et al., 2004), leading to an increased number of mucus-producing cells and eventually mucus hypersecretion. There is an inverse relationship between goblet cell metaplasia and FEV1, a measure of lung function (Shao et al., 2004).

Ozone and endotoxin induce mucus cell metaplasia in rat airway epithelium (Harkema and Hotchkiss, 1993)


Increased EGFR expression is associated with bronchial metaplasia (Kurie et al., 1996).

How it is Measured or Detected

Methods that have been previously reviewed and approved by a recognized authority should be included in the Overview section above. All other methods, including those well established in the published literature, should be described here. Consider the following criteria when describing each method: 1. Is the assay fit for purpose? 2. Is the assay directly or indirectly (i.e. a surrogate) related to a key event relevant to the final adverse effect in question? 3. Is the assay repeatable? 4. Is the assay reproducible?

In vitro/in vivo experiments

Increased mucus is detected using Alcian blue/periodic acid-Schiff (AB/PAS) for detection of mucin glycoproteins. In cell and animal experiments, literature equates an increase in mucus production with "mucus metaplasia" even though the study did not look at the origin of cells to see if they were differentiated from non-goblet cells.

Clinical

Evidence Supporting Taxonomic Applicability

References