Event:920
Contents
Event Title
Key Event Overview
Please follow link to widget page to edit this section.
If you manually enter text in this section, it will get automatically altered or deleted in subsequent edits using the widgets.
AOPs Including This Key Event
AOP Name | Event Type | Essentiality |
---|---|---|
EGFR Activation Leading to Mucus Hypersecretion | KE | Moderate |
Taxonomic Applicability
Name | Scientific Name | Evidence | Links |
---|
Level of Biological Organization
Biological Organization |
---|
How this Key Event works
Metaplasia is the replacement of a differentiated cell type with another differentiated cell type. In the case of goblet cell metaplasia, ciliated or Clara cells transdifferentiate into goblet cells (Tyner et al., 2006), (Evans et al., 2004), leading to an increased number of mucus-producing cells and eventually mucus hypersecretion. There is an inverse relationship between goblet cell metaplasia and FEV1, a measure of lung function (Shao et al., 2004).
Ozone and endotoxin induce mucus cell metaplasia in rat airway epithelium (Harkema and Hotchkiss, 1993)
Increased EGFR expression is associated with bronchial metaplasia (Kurie et al., 1996).
How it is Measured or Detected
Methods that have been previously reviewed and approved by a recognized authority should be included in the Overview section above. All other methods, including those well established in the published literature, should be described here. Consider the following criteria when describing each method: 1. Is the assay fit for purpose? 2. Is the assay directly or indirectly (i.e. a surrogate) related to a key event relevant to the final adverse effect in question? 3. Is the assay repeatable? 4. Is the assay reproducible?
In vitro/in vivo experiments
Increased mucus is detected using Alcian blue/periodic acid-Schiff (AB/PAS) for detection of mucin glycoproteins. In cell and animal experiments, literature equates an increase in mucus production with "mucus metaplasia" even though the study did not look at the origin of cells to see if they were differentiated from non-goblet cells.
Clinical