Difference between revisions of "Relationship:992"

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(References)
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Barbosa, V.A., Luciano, T.F., Marques, S.O., Vitto, M.F., Souza, D.R., Silva, L.A., Santos, J.P.A., Moreira, J.C., Dal-Pizzol, F., Lira, F.S., et al. (2013). Acute exercise induce endothelial nitric oxide synthase phosphorylation via Akt and AMP-activated protein kinase in aorta of rats: Role of reactive oxygen species. Int. J. Cardiol. 167, 2983–2988.
 +
 
Chen, X., Xu, J., Feng, Z., Fan, M., Han, J., and Yang, Z. (2010). Simvastatin combined with nifedipine enhances endothelial cell protection by inhibiting ROS generation and activating Akt phosphorylation. Acta Pharmacol. Sin. 31, 813–820.
 
Chen, X., Xu, J., Feng, Z., Fan, M., Han, J., and Yang, Z. (2010). Simvastatin combined with nifedipine enhances endothelial cell protection by inhibiting ROS generation and activating Akt phosphorylation. Acta Pharmacol. Sin. 31, 813–820.
  
 
Dhar, A., Dhar, I., Desai, K.M., and Wu, L. (2010). Methylglyoxal scavengers attenuate endothelial dysfunction induced by methylglyoxal and high concentrations of glucose. Br. J. Pharmacol. 161, 1843–1856.
 
Dhar, A., Dhar, I., Desai, K.M., and Wu, L. (2010). Methylglyoxal scavengers attenuate endothelial dysfunction induced by methylglyoxal and high concentrations of glucose. Br. J. Pharmacol. 161, 1843–1856.
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Du, J., Fan, L.M., Mai, A., and Li, J.-M. (2013a). Crucial roles of Nox2-derived oxidative stress in deteriorating the function of insulin receptors and endothelium in dietary obesity of middle-aged mice. Br. J. Pharmacol. 170, 1064–1077.
 +
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Du, X.L., Edelstein, D., Dimmeler, S., Ju, Q., Sui, C., and Brownlee, M. (2001). Hyperglycemia inhibits endothelial nitric oxide synthase activity by posttranslational modification at the Akt site. J. Clin. Invest. 108, 1341–1348.
 +
 +
Guterbaum, T.J., Braunstein, T.H., Fossum, A., Holstein-Rathlou, N.-H., Torp-Pedersen, C.T., and Domínguez, H. (2013). Endothelial nitric oxide synthase phosphorylation at Threonine 495 and mitochondrial reactive oxygen species formation in response to a high H₂O₂ concentration. J. Vasc. Res. 50, 410–420.
 +
 +
Hu, Z., Chen, J., Wei, Q., and Xia, Y. (2008). Bidirectional actions of hydrogen peroxide on endothelial nitric-oxide synthase phosphorylation and function: co-commitment and interplay of Akt and AMPK. J. Biol. Chem. 283, 25256–25263.
 +
 +
Kumar, S., Sud, N., Fonseca, F.V., Hou, Y., and Black, S.M. (2010). Shear stress stimulates nitric oxide signaling in pulmonary arterial endothelial cells via a reduction in catalase activity: role of protein kinase C delta. Am. J. Physiol. Lung Cell. Mol. Physiol. 298, L105–L116.
 +
 +
Song, P., Wu, Y., Xu, J., Xie, Z., Dong, Y., Zhang, M., and Zou, M.-H. (2007). Reactive nitrogen species induced by hyperglycemia suppresses Akt signaling and triggers apoptosis by upregulating phosphatase PTEN (phosphatase and tensin homologue deleted on chromosome 10) in an LKB1-dependent manner. Circulation 116, 1585–1595.
 +
 +
Song, P., Xie, Z., Wu, Y., Xu, J., Dong, Y., and Zou, M.-H. (2008). Protein kinase Czeta-dependent LKB1 serine 428 phosphorylation increases LKB1 nucleus export and apoptosis in endothelial cells. J. Biol. Chem. 283, 12446–12455.
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Zhang, W., Han, Y., Meng, G., Bai, W., Xie, L., Lu, H., Shao, Y., Wei, L., Pan, S., Zhou, S., et al. (2014). Direct renin inhibition with aliskiren protects against myocardial ischemia/reperfusion injury by activating nitric oxide synthase signaling in spontaneously hypertensive rats. J. Am. Heart Assoc. 3, e000606.
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 +
Zou, M.-H., Hou, X.-Y., Shi, C.-M., Nagata, D., Walsh, K., and Cohen, R.A. (2002). Modulation by peroxynitrite of Akt- and AMP-activated kinase-dependent Ser1179 phosphorylation of endothelial nitric oxide synthase. J. Biol. Chem. 277, 32552–32557.

Revision as of 01:39, 22 April 2016


Key Event Relationship Overview

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Description of Relationship

Upstream Event Downstream Event/Outcome
Oxidative Stress, Increase Akt/eNOS activity, Decrease

AOPs Referencing Relationship

AOP Name Type of Relationship Weight of Evidence Quantitative Understanding
Oxidative Stress Leading to Arterial Stiffness Directly Leads to Strong Moderate

Taxonomic Applicability

Name Scientific Name Evidence Links

How Does This Key Event Relationship Work

Exposure to known inducers of oxidative stress causes the phosphorylation of Akt and eNOS, leading to a decrease in their activities. However, hydrogen peroxide has been reported to have conflicting effects depending on the dose and time (Hu et al., 2008).

Weight of Evidence

Biological Plausibility

Multiple experimental studies reported modulations in Akt and eNOS phosphorylation/activity following exposure to oxidative stress, thus providing strong biological plausibility for this key event relationship.

In human umbilical vein endothelial cells (HUVECs), peroxynitrite significantly inhibited Akt phosphorylation at Ser473 and Akt activity (Song et al., 2007, 2008; Zou et al., 2002). However, Zou et al. found that peroxynitrite increase eNOS phosphorylation at Ser1199, but decreased NO, suggesting eNOS phosphorylation may not dependent on Akt. Treatment of BAECs with SIN-1, a source of peroxynitrite anion, inhibited eNOS activity by 30% and Akt/eNOS phosphorylation (Das et al., 2014).

High glucose can also inhibit Akt phosphorylation (Song et al., 2008), which supports other studies in which eNOS phosphorylation was affected by glucose. HUVECs treated with methylglyoxal and high glucosed reduced eNOS bradyinin-stimulated eNOS activity via Ser1177 phosphorylation (Dhar et al., 2010), while hyperglycemia inhibited eNOS activity in bovine aortic endothelial cells (BAECs) (Du et al., 2001). In EA.hy926 endothelial cells, methylglyoxal reduced eNOS phosphorylation at Ser1177 (Su et al., 2013). High-fat diet wild-type mice had decreased Akt and eNOS phosphorylation compared to normal chow diet wild-type mice (Du et al., 2013).

Cigarette smoke extract (CSE) also inhibited Akt and eNOS in VEGF-stimulated HUVECs (Michaud et al., 2006). Myocardial ischemia decreased phosphorylated AKT and eNOS in spontaneously hypertension (SHR) rats compared to sham animals (Zhang et al., 2014).

Empirical Support for Linkage

Include consideration of temporal concordance here

There is moderate empirical support for this link as eNOS activity and reactive oxygen species (ROS) were modulated to several stressors. Methylglyoxal (30 μM) and high glucose (25 mM) for 24 hours caused an increase in ROS (control: 0.75, MG: 1.25, HG: 1) and decrease in eNOS activity (control: 100%, MG: 50%, HG: 60%) in HUVECs (Dhar et al., 2010). SIN-1 treatment for two hours increased ROS (100% to 300%) and decreased eNOS activity (100% to 70%) (Das et al., 2014). The increase in ROS and decrease in eNOS after H2O2 (100 μmol/L) was reversed with the addition of simvastatin (1 μmol/L) and nifedipine (1 μmol/L) (Chen et al., 2010).

Uncertainties or Inconsistencies

There are many studies examining the effect of hydrogen peroxide (H2O2) on Akt/eNOS phosphorylation, but there are conflicting results. One study showed that H2O2 initially increased eNOS Ser1179 phosphorylation and activity but after the peak increase at 30 minutes, eNOS Ser1179 phosphorylation dramatically decreased (Hu et al., 2008). A similar trend was observed for Akt phosphorylation. However, one study that exposed cells to H2O2 for 30 minutes showed increased Akt/eNOS phosphorylation, but its H2O2 concentration was much higher 200 μM compared to 5 μM (Barbosa et al., 2013). Other studies include H2O2 (100 μM/L, 30 min) inhibited eNOS expression (Chen et al., 2010), H2O2 (400 uM, 30 min) increased eNOS phosphorylation at the inhibitory site Thr495 (Guterbaum, 2013), and H2O2 increasing eNOS phosphorylation at Ser1177 (Kumar et al., 2010). Due to these inconsistencies, H2O2 may not be applicable to this key event relationship.

Quantitative Understanding of the Linkage

Is it known how much change in the first event is needed to impact the second? Are there known modulators of the response-response relationships? Are there models or extrapolation approaches that help describe those relationships?

Dhar et al. showed that an increase of >30% in ROS from high glucose and methylglyoxal was able to decrease eNOS activity, while Das et al. showed a three-fold increase in ROS lead to a 30% reduction in eNOS activity. SIN-1, high glucose, methylglyoxal and H2O2 were demonstrated to cause a modulation in both key events at the same time.

Evidence Supporting Taxonomic Applicability

The relationship between oxidative stress and decreased Akt/eNOS activity is supported by studies performed in humans, cows, mice and rats.

References

Barbosa, V.A., Luciano, T.F., Marques, S.O., Vitto, M.F., Souza, D.R., Silva, L.A., Santos, J.P.A., Moreira, J.C., Dal-Pizzol, F., Lira, F.S., et al. (2013). Acute exercise induce endothelial nitric oxide synthase phosphorylation via Akt and AMP-activated protein kinase in aorta of rats: Role of reactive oxygen species. Int. J. Cardiol. 167, 2983–2988.

Chen, X., Xu, J., Feng, Z., Fan, M., Han, J., and Yang, Z. (2010). Simvastatin combined with nifedipine enhances endothelial cell protection by inhibiting ROS generation and activating Akt phosphorylation. Acta Pharmacol. Sin. 31, 813–820.

Dhar, A., Dhar, I., Desai, K.M., and Wu, L. (2010). Methylglyoxal scavengers attenuate endothelial dysfunction induced by methylglyoxal and high concentrations of glucose. Br. J. Pharmacol. 161, 1843–1856.

Du, J., Fan, L.M., Mai, A., and Li, J.-M. (2013a). Crucial roles of Nox2-derived oxidative stress in deteriorating the function of insulin receptors and endothelium in dietary obesity of middle-aged mice. Br. J. Pharmacol. 170, 1064–1077.

Du, X.L., Edelstein, D., Dimmeler, S., Ju, Q., Sui, C., and Brownlee, M. (2001). Hyperglycemia inhibits endothelial nitric oxide synthase activity by posttranslational modification at the Akt site. J. Clin. Invest. 108, 1341–1348.

Guterbaum, T.J., Braunstein, T.H., Fossum, A., Holstein-Rathlou, N.-H., Torp-Pedersen, C.T., and Domínguez, H. (2013). Endothelial nitric oxide synthase phosphorylation at Threonine 495 and mitochondrial reactive oxygen species formation in response to a high H₂O₂ concentration. J. Vasc. Res. 50, 410–420.

Hu, Z., Chen, J., Wei, Q., and Xia, Y. (2008). Bidirectional actions of hydrogen peroxide on endothelial nitric-oxide synthase phosphorylation and function: co-commitment and interplay of Akt and AMPK. J. Biol. Chem. 283, 25256–25263.

Kumar, S., Sud, N., Fonseca, F.V., Hou, Y., and Black, S.M. (2010). Shear stress stimulates nitric oxide signaling in pulmonary arterial endothelial cells via a reduction in catalase activity: role of protein kinase C delta. Am. J. Physiol. Lung Cell. Mol. Physiol. 298, L105–L116.

Song, P., Wu, Y., Xu, J., Xie, Z., Dong, Y., Zhang, M., and Zou, M.-H. (2007). Reactive nitrogen species induced by hyperglycemia suppresses Akt signaling and triggers apoptosis by upregulating phosphatase PTEN (phosphatase and tensin homologue deleted on chromosome 10) in an LKB1-dependent manner. Circulation 116, 1585–1595.

Song, P., Xie, Z., Wu, Y., Xu, J., Dong, Y., and Zou, M.-H. (2008). Protein kinase Czeta-dependent LKB1 serine 428 phosphorylation increases LKB1 nucleus export and apoptosis in endothelial cells. J. Biol. Chem. 283, 12446–12455.

Zhang, W., Han, Y., Meng, G., Bai, W., Xie, L., Lu, H., Shao, Y., Wei, L., Pan, S., Zhou, S., et al. (2014). Direct renin inhibition with aliskiren protects against myocardial ischemia/reperfusion injury by activating nitric oxide synthase signaling in spontaneously hypertensive rats. J. Am. Heart Assoc. 3, e000606.

Zou, M.-H., Hou, X.-Y., Shi, C.-M., Nagata, D., Walsh, K., and Cohen, R.A. (2002). Modulation by peroxynitrite of Akt- and AMP-activated kinase-dependent Ser1179 phosphorylation of endothelial nitric oxide synthase. J. Biol. Chem. 277, 32552–32557.