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Key Event Title
|Level of Biological Organization|
Key Event Components
Key Event Overview
AOPs Including This Key Event
|Homo sapiens||Homo sapiens||High||NCBI|
|All life stages||High|
Key Event Description
Upon the Wnt signaling activation, beta-catenin is stabilized and activated via inhibition of the phosphorylation by GSK3beta (Huang et al., 2019). Once the beta-catenin is stabilized, it translocates into the nucleus and enhance the expression of target genes of Wnt/beta-catenin signaling pathway (Huang et al., 2019). Beta-catenin activation is related to cancer(Tanabe, 2014).
How It Is Measured or Detected
•The beta-catenin level in nucleus is measured by immunoblotting with anti-beta-catenin antibody (Huang et al., 2019)
•The beta-catenin nuclear translocation is measured by immunofluorescence assay (Huang et al., 2019).
•Activity of beta-catenin is measured by Wnt/beta-catenin activity assay, in which the vector containing the firefly luciferase gene controlled by TCF/LEF binding sites is transfected in the cells (Naujok et al., 2014).
Domain of Applicability
•Beta-catenin is stabilized and translocated into nucleus in Homo sapiens (Huang et al., 2019).
•Beta-catenin is activated in Homo sapiens (Huang et al., 2019) (Naujok et al., 2014).
Evidence for Perturbation by Stressor
CHIR-99021 activates beta-catenin (Naujok et al., 2014).
CHIR-98014 activates beta-catenin (Naujok et al., 2014).
Huang, J. Q., Wei, F. K., Xu, X. L., Ye, S. X., Song, J. W., Ding, P. K., . . . Gong, L. Y. (2019). SOX9 drives the epithelial-mesenchymal transition in non-small-cell lung cancer through the Wnt/beta-catenin pathway. J Transl Med, 17(1), 143. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/31060551. doi:10.1186/s12967-019-1895-2
Naujok, O., Lentes, J., Diekmann, U., Davenport, C., & Lenzen, S. (2014). Cytotoxicity and activation of the Wnt/beta-catenin pathway in mouse embryonic stem cells treated with four GSK3 inhibitors. BMC Res Notes, 7, 273. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/24779365. doi:10.1186/1756-0500-7-273
Tanabe, S. (2014). Role of mesenchymal stem cells in cell life and their signaling. World journal of stem cells, 6(1), 24-32. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/24567785