Comments for Relationship:361
Karen Watanabe
On July 13, 2023 22:07
To current KER description coauthors,
The revised KER description that you reviewed in June 2023 inadvertently omitted a few studies that we would like to add to the Empirical Evidence table. Please email karen.watanabe@asu.edu with your approval, questions or concerns.
| Stressor | Experimental Model | Tested Concentrations | Exposure route | Exposure duration | Overactivation of NMDAR (KE up) (measurements, quantitative if available) | Increased intracelllular Ca 2+ levels (KE down) (measurements, quantitative if available) | Refereences | Temporal Relationship | Dose-response relationship | Incidence | Comments |
|
Glutamate, NMDA |
Mouse cortical astrocytes |
Glutamate: 100 µM |
|
Brief (1s application) |
|
Increased intracellular Ca2+ measured through Fluo-3 Flourescence |
Palygin et al., 2011 |
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Provides time-series data of intracellular calcium measured through fluoresence given an application of Glu, NMDA, or Glu + D-AP5 in mouse cortical astrocytes. Cells were additionally exposed to D-AP5, an NMDA antagonist, and showed reduced fluorscence changes. |
|
Glutamate |
Cultured rat hippocampal neurons |
500 µM |
|
Time course (0 to 45 minutes) |
|
Increased intracellular Ca2+ measured through Fura-2 Flourescence |
Michaels and Rothman 1990 |
|
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Provides time-series data of intracellular calcium measured through fluoresence, as well as directly providing calculated intracellular calcium concentrations in response to high concentrations of applied Glu, both alone and with antagonists. |
|
NMDA, Glutamate |
Neocortical neurons of Swiss-Webster mice |
Glutamate: 300 µM |
|
Time course (0 to 20 minutes) and (0 to 2 minutes) |
|
Increased intracellular Ca2+ measured through Fura-2/AM, Fura-2/K+, Fura-2/dextran, BTC |
Hyrc et al., 1997 |
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Provides time-series data of intracellular calcium measured through a variety of fluorsence calcium indicators given an application of the selective agonist NMDA. |
|
Glutamate |
Computational model (CA1 pyramidal neuron) |
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|
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Models the concentration of Ca2+ in spine(s) of neuron |
Hu et al., 2018 |
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Developed a computational model of a glutamatergic spine which models intracellular calcium dynamics and sources of calcium influx including activation of NMDA receptors. |
Karen Watanabe
On July 11, 2023 23:13
Comment by S. Tanabe upon review of revised KER description. Text in black is from the current description; red text is text to add.
|
DomA |
Adult male Sprague Dawley rats |
10 µM [ST1]
|
Brain microinjection |
24 h after microinjection |
|
Increased phosphorylated CaMKII and phosphorylated CREB levels in hippocampus |
Qiu and Currás-Collazo, 2006 |
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[ST1]It seems that the authors also tested concentrations below 10 microM, according to the abstract.
Karen Watanabe
On July 11, 2023 23:00
From S. Tanabe (review of KER description).
Text in KER description
Glufosinate (GLF) is the methylphosphinate analog of glutamate that directly can activate NMDARs (Lantz et al., 2014, Matsumura et al., 2001, Faro et al., 2013) (as described in KE: NMDARs, Binding of agonist[ST1] ). It is well established in the existing literature that activation of NMDARs leads to the intra-cellular Ca2+ overload and based on this assumption it can be suggested that an exposure to GLF leads to increased intra-cellular calcium levels.
[ST1]Is this KE875 “Ionotropic glutamate receptors, Binding of agonist” ? The description of GLF exist in AOPWiki https://aopwiki.org/wiki/index.php/Event:875, however, it is not seen in the current AOP-Wiki
https://aopwiki.org/events/875.
KE387 “Binding of agonist, NMDARs” seems to have no description on GLF https://aopwiki.org/events/387
edited - July 11, 2023 23:14