API

Relationship: 442

Title

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Inhibition, Na+/I- symporter (NIS) leads to Thyroidal Iodide, Decreased

Upstream event

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Inhibition, Na+/I- symporter (NIS)

Downstream event

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Thyroidal Iodide, Decreased

Key Event Relationship Overview

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AOPs Referencing Relationship

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AOP Name Directness Weight of Evidence Quantitative Understanding
Sodium Iodide Symporter (NIS) Inhibition and Subsequent Adverse Neurodevelopmental Outcomes in Mammals directly leads to Strong Strong
Inhibition of Na+/I- symporter (NIS) leads to learning and memory impairment directly leads to Strong Strong

Taxonomic Applicability

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Term Scientific Term Evidence Link
human Homo sapiens Strong NCBI
rat Rattus norvegicus Strong NCBI
mouse Mus musculus Strong NCBI
Xenopus laevis laevis Xenopus laevis laevis Moderate NCBI

Sex Applicability

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Sex Evidence
Mixed Strong

Life Stage Applicability

?

Term Evidence
During brain development Strong

How Does This Key Event Relationship Work

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NIS is a membrane protein implicated in iodide uptake into the follicular cells of the thyroid. Other large anions can be also bound by NIS and inhibit accumulation of iodide into the thyroid by competing binding with iodide (Wolff, 1964).

Weight of Evidence

?


Many studies have shown inhibition of radioactive iodide uptake by using different cell models and assays. However, there have been identified only few specific NIS inhibitors up to date, while all the others are thought to act through different inhibitory mechanisms. Monovalent anions, others than iodide, are also transported by NIS but Nitrate (NO3-), thiocyanate (SCN-), perchlorate (ClO-4), dysidenin and aryltrifluoroborates are of particular dietary and environmental importance (Jones et al., 1996; Tonacchera et al., 2004; De Groef et al., 2006).

Recent revision of the affinity constant for perchlorate binding to the NIS symporter based on in vitro and human in vivo data, performed by refitting published in vitro data, in which perchlorate-induced inhibition of iodide uptake via the NIS was measured, yielding a Michaelis-Menten kinetic constant (Km) of 1.5 μm, showed that a 60% lower value for the Km, equal to 0.59 μm. Substituting this value into the PBPK model for an average adult human significantly improved model agreement with the human RAIU data for exposures <100 μg kg-1 day-1 (Schlosser PM, 2016).

 

There are many studies showing the effect of inhibition of NIS on thyroidal iodide uptake:

- Cianchetta et al., 2010 For this study the rat FRTL5 thyroid cell line endogenously expressing NIS, and the monkey kidney fibroblast-like cells (COS-7) transfected with hNIS were used. NIS functionality was assessed with the use of the Yellow Fluorescent Protein (YFP) variant YFP-H148Q/I152L, a genetically encodable biosensor of intracellular perchlorate concentration monitored by real-time fluorescence microscopy. Decrease of YFP-H148Q/I152L fluorescence in FRTL-5 cells occurs as a result of NIS-mediated uptake and binding to the intracellular fluorochrome (Rhoden et al., 2007). The biosensor was used to compare the kinetics of iodide and perchlorate transport by NIS, and to assess the ability of perchlorate to inhibit iodide transport. Additionally, perchlorate was shown to inhibit NIS function (competitive inhibition) by preventing iodide-induced changes in fluorescence of FRTL5 cells. Perchlorate caused a concentration-dependent inhibition of iodide uptake in the initial influx rate (IC50=1.6μM) and in the intracellular concentration of iodide (IC50=1.1μM). Also, both perchlorate and iodide (1–1000 μM) induced concentration-dependent decreases in YFP-H148Q/I152L fluorescence in COS-7 cells expressing hNIS, but had no effect (< 2%) in COS-7 cells lacking hNIS. Additionally, perchlorate induced a significantly smaller decrease in fluorescence (10.6% at 1 mM) than iodide (31.8% at 1 mM iodide). Thus, it was confirmed that the reduction of fluorescence was due to NIS-mediated anion transport into the cells, excluding non-specific effects.

 

- Tonacchera et al., 2004 Chinese hamster ovary (CHO) cell line had been stably transfected with human NIS and the measurement of iodide uptake was performed with the use of radioactive iodide uptake (RAIU) method. It was shown that the inhibition of iodide uptake was dose-dependent when using the known NIS inhibitors (ClO-4, NO3-, SCN-). Additionally, unlabeled I- (non 125I) was used to investigate the inhibition level of radioiodide uptake and to compare it with the potency of the other monoions, which are known NIS inhibitors. The IC50 values for the studied monoions were the following: ClO4-: IC50 was 1.22 μΜ; SCN-: IC50 was 18.7 μΜ; NO3-: IC50 was 293 μΜ; I-: IC50 was 36.6 μΜ. Finally, the present study investigated the joint effects of simultaneous exposure to multiple RAIU inhibitors, by generating multiple dose-response curves in the presence of fixed concentrations of inhibitors. The results of those experiments indicated a competition between the four anions with similar size for access to the binding sites of the NIS. The prediction model developed in this study, actually suggests that thyroidal iodide uptake is approximately proportional to iodide nutrition for any fixed inhibitor concentration, answering to the question whether dietary iodide can modulate the inhibitory effects of the known environmental goitrogens.

- Waltz et al., 2010 Measurement of iodide uptake was performed with a non-radioactive method. By using the rat thyroid low serum 5 (FRTL5) cells, which endogenously express NIS, a spectrophotometric assay was developed and the iodide accumulation was determined based on the catalytic reduction of yellow cerium to colorless cerium in the presence of arsenious acid (Sandell-Kolthoff reaction). A dose-dependent inhibition of iodide uptake was shown. The IC50 values for the studied compounds were the following: Sodium perchlorate (NaClO4): IC50 was 0.1 μΜ; Sodium thiocyanate (NaSCN): IC50 was 12 μΜ; Sodium nitrate (NaNO3): IC50 was 800 μΜ; Sodium Tetrafluoroborate (NaBF4): IC50 was 1.2 μΜ.

- Lecat-Guillet et al., 2007; 2008a A fully automated radioiodide uptake assay was developed and some known NIS inhibitors were tested. A dose-dependent inhibition of iodide uptake was shown. The IC50 values for the studied compounds were the following: Sodium perchlorate (NaClO4): IC50 was 1 μΜ; Sodium thiocyanate (NaSCN): IC50 was 14 μΜ; Sodium nitrate (NaNO3): IC50 was 250 μΜ; Sodium Tetrafluoroborate (NaBF4): IC50 was 0.75 μΜ.  Additionally, a library of 17020 compounds was screened for the identification of new human NIS inhibitors. The identification was based on the magnitude of changes in iodide uptake using Human Embryonic Kidney 293 (HEK293) cells, stably transfected with the hNIS. The same experiments and with similar results were also performed in rat thyroid derived cells (FRTL5), which endogenously express NIS. Compounds that inhibited iodide uptake in a time-dependent manner were considered to act through direct NIS inhibition. In contrast, those compounds that had a delayed effect on iodide uptake were thought to act through a sodium gradient disruption system resulting in indirect inhibition of iodide transport. Perchlorate was used as a positive control in these experiments and, as expected, it blocked iodide uptake immediately and totally throughout the experiment. Dysidenin was also used as a control and the IC50 value identified was 2 μΜ. All the compounds that were used for these experiments were small drug-like molecules that have not been detected in the environment and they were named as ITBs (Iodide Transport Blockers).

- Lecat-Guillet et al., 2008b With the same fully automated radioiodide uptake assay, as described above, new NIS inhibitors were also identified. The organotrifluoroborate (BF3−) was found to inhibit iodide uptake with an IC50 value of 0.4 μM using rat-derived thyroid cells (FRTL5). The biological activity is rationalized by the presence of the ion BF3− as a minimal binding motif for substrate recognition at the iodide binding site.

- Lindenthal et al., 2009 With the use of a patch-clamp technique an analysis of the NIS inhibitors identified by Lecat-Guillet et al., 2008 (named ITB-1 to ITB-10 for "Iodide Transport Blockers") was evaluated in Xenopus oocytes expressing NIS to further assess the inhibitory effect of those molecules specifically on NIS activity. Four of those molecules (ITB-3, ITB-9, ITB-5 and ITB-4) were identified as the most potent, non-competitive NIS inhibitors. The effects of dysidenin were also analyzed with the same technique, as it had been reported to be a specific inhibitor of NIS (Vroye et al., 1998). It was found that dysidenin (50 μM) induced a rapid and reversible inhibition of the iodide (about 40%) of induced current in mNIS-expressing oocytes, but did not evoke any currents in the absence of iodide, suggesting that this effect was due to the inhibition of NIS activity.

- Greer et al., 2002 In human studies, potassium perchlorate was used to predict inhibition of thyroidal iodide uptake by applying the RAIU method. Greer et al., tested body weight adjusted doses of potassium perchlorate and an assessment of RAIU uptake was performed on day 2 and day 14 of treatment and 24 h following treatment termination (on day 15). The NOEL value for inhibition of thyroidal uptake was 0.007 mg/kg-day, while the true NEL value was estimated to be 0.0052 and 0.0064 mg/kg-day. According to the dose-response inhibition of iodide uptake the maximum percentage of iodide inhibition at the doses of 0.0052 and 0.0064 mg/kg-day is 8.3-9.5%, which is physiologically insignificant for a person with dietary sufficient iodine intake.

- Wen et al., 2016 By using human MCF-7 cells, a breast adenocarcinoma cell line, which express inducible NIS in the presence of all-trans retinoic acid (ATRA) it has been shown that inhibition of sterol regulatory element-binding proteins (SREBP) maturation by treatment with 25-hydroxycholesterol (5 µM) for 48 hr reduced ATRA (1 µM)-induced mRNA concentration of NIS and decreased iodide uptake by approximately 20%. This study showed for the first time that the NIS gene and iodide uptake are regulated by SREBP in cultured human mammary epithelial cells.

- Arriagada et al. 2015 This study showed that 2 hr or 5 hr exposure to excess I- (100 μM) respectively in FRTL-5 cells and in ex-vivo rat thyroid gland (removed after single in vivo i.p. injection of 100 μg of I in 500 μL of distilled water, and analysis of 125I thyroid uptake), induced inhibition of I- uptake through the NIS (~ 30% uptake inhibition after 5 hr in vivo), a process known as the Wolff-Chaikoff effect, which was not associated with a decrease of NIS expression or a change in NIS localization. Incubation of FRTL-5 cells with excess I- for 2 hr increased hydrogen peroxide generation. Also incubation with hydrogen peroxide (100 μM) decreased NIS-mediated I- transport, effect that was reverted by ROS scavengers.

 

 

The thyroid system is quite complex and therefore some inconsistent results have been produced by recent studies. For example, it has been observed in healthy volunteers that a 6-month exposure to perchlorate at doses up to 3 mg/d (low doses) had no effect on thyroid function, including inhibition of thyroid iodide uptake as well as serum levels of thyroid hormones, TSH, and Tg (Braverman et al., 2006). However, this study was limited by the small sample size and is obviously statistically underpowered.

Biological Plausibility

?

NIS is a membrane bound glycoprotein and its main physiological function is to transport one iodide ion along with two sodium ions across the basolateral membrane of thyroid follicular cells. It uses the sodium gradient generated by the Na+/K+ ATPase for the active transport of iodide into the thyrocytes (Eskandari et al., 1997). Extensive studies on NIS protein have identified 14 different mutations and each one of them is related to Iodine Transport Deficiencies (ITD) (reviewed in Spitzweg and Morris, 2010). Most of these mutations have been characterized and it is well known that they even lead to the synthesis of truncated protein (Pohlenz et al., 1997; Pohlenz et al., 1998), partial deletions (Kosugi et al., 2002; Tonacchera et al., 2003; Montanelli et al., 2009) or substitutions of amino acids (Matsuda and Kosugi, 1997; Kosugi et al., 1999; Szinnai et al., 2006) that eventually result in total or partial NIS dysfunction. While most of the NIS mutants have been further investigated and the functional relationship between the NIS dysfunction and ITD is well established (reviewed in Darrouzet et al., 2014; Portulano et al., 2014), the exact structural relationship between mutated NIS and ITD still needs to be elucidated and the molecular modelling of the protein would greatly benefit these studies.

Recent revision of the affinity constant for perchlorate binding to the NIS symporter based on in vitro and human in vivo data, performed by refitting published in vitro data, in which perchlorate-induced inhibition of iodide uptake via the NIS was measured, yielding a Michaelis-Menten kinetic constant (Km) of 1.5 μm, showed that a 60% lower value for the Km, equal to 0.59 μm. Substituting this value into the PBPK model for an average adult human significantly improved model agreement with the human RAIU data for exposures <100 μg kg-1 day-1 (Schlosser PM, 2016).

Empirical Support for Linkage

?

Many studies have shown inhibition of radioactive iodide uptake by using different cell models and assays. However, there have been identified only few specific NIS inhibitors up to date, while all the others are thought to act through different inhibitory mechanisms. Monovalent anions, others than iodide, are also transported by NIS but Nitrate (NO3-), thiocyanate (SCN-), perchlorate (ClO-4), dysidenin and aryltrifluoroborates are of particular dietary and environmental importance (Jones et al., 1996; Tonacchera et al., 2004; De Groef et al., 2006).

Recent revision of the affinity constant for perchlorate binding to the NIS symporter based on in vitro and human in vivo data, performed by refitting published in vitro data, in which perchlorate-induced inhibition of iodide uptake via the NIS was measured, yielding a Michaelis-Menten kinetic constant (Km) of 1.5 μm, showed that a 60% lower value for the Km, equal to 0.59 μm. Substituting this value into the PBPK model for an average adult human significantly improved model agreement with the human RAIU data for exposures <100 μg kg-1 day-1 (Schlosser PM, 2016).

There are many studies showing the effect of inhibition of NIS on thyroidal iodide uptake:

- Cianchetta et al., 2010 For this study the rat FRTL5 thyroid cell line endogenously expressing NIS, and the monkey kidney fibroblast-like cells (COS-7) transfected with hNIS were used. NIS functionality was assessed with the use of the Yellow Fluorescent Protein (YFP) variant YFP-H148Q/I152L, a genetically encodable biosensor of intracellular perchlorate concentration monitored by real-time fluorescence microscopy. Decrease of YFP-H148Q/I152L fluorescence in FRTL-5 cells occurs as a result of NIS-mediated uptake and binding to the intracellular fluorochrome (Rhoden et al., 2007). The biosensor was used to compare the kinetics of iodide and perchlorate transport by NIS, and to assess the ability of perchlorate to inhibit iodide transport. Additionally, perchlorate was shown to inhibit NIS function (competitive inhibition) by preventing iodide-induced changes in fluorescence of FRTL5 cells. Perchlorate caused a concentration-dependent inhibition of iodide uptake in the initial influx rate (IC50=1.6μM) and in the intracellular concentration of iodide (IC50=1.1μM). Also, both perchlorate and iodide (1–1000 μM) induced concentration-dependent decreases in YFP-H148Q/I152L fluorescence in COS-7 cells expressing hNIS, but had no effect (< 2%) in COS-7 cells lacking hNIS. Additionally, perchlorate induced a significantly smaller decrease in fluorescence (10.6% at 1 mM) than iodide (31.8% at 1 mM iodide). Thus, it was confirmed that the reduction of fluorescence was due to NIS-mediated anion transport into the cells, excluding non-specific effects.

- Tonacchera et al., 2004 Chinese hamster ovary (CHO) cell line had been stably transfected with human NIS and the measurement of iodide uptake was performed with the use of radioactive iodide uptake (RAIU) method. It was shown that the inhibition of iodide uptake was dose-dependent when using the known NIS inhibitors (ClO-4, NO3-, SCN-). Additionally, unlabeled I- (non 125I) was used to investigate the inhibition level of radioiodide uptake and to compare it with the potency of the other monoions, which are known NIS inhibitors. The IC50 values for the studied monoions were the following: ClO4-: IC50 was 1.22 μΜ; SCN-: IC50 was 18.7 μΜ; NO3-: IC50 was 293 μΜ; I-: IC50 was 36.6 μΜ. Finally, the present study investigated the joint effects of simultaneous exposure to multiple RAIU inhibitors, by generating multiple dose-response curves in the presence of fixed concentrations of inhibitors. The results of those experiments indicated a competition between the four anions with similar size for access to the binding sites of the NIS. The prediction model developed in this study, actually suggests that thyroidal iodide uptake is approximately proportional to iodide nutrition for any fixed inhibitor concentration, answering to the question whether dietary iodide can modulate the inhibitory effects of the known environmental goitrogens.

- Waltz et al., 2010 Measurement of iodide uptake was performed with a non-radioactive method. By using the rat thyroid low –serum 5 (FRTL5) cells, which endogenously express NIS, a spectrophotometric assay was developed and the iodide accumulation was determined based on the catalytic reduction of yellow cerium to colorless cerium in the presence of arsenious acid (Sandell-Kolthoff reaction). A dose-dependent inhibition of iodide uptake was shown. The IC50 values for the studied compounds were the following: Sodium perchlorate (NaClO4): IC50 was 0.1 μΜ Sodium thiocyanate (NaSCN): IC50 was 12 μΜ Sodium nitrate (NaNO3): IC50 was 800 μΜ Sodium Tetrafluoroborate (NaBF4): IC50 was 1.2 μΜ

- Lecat-Guillet et al., 2007; 2008a A fully automated radioiodide uptake assay was developed and some known NIS inhibitors were tested. A dose-dependent inhibition of iodide uptake was shown. The IC50 values for the studied compounds were the following: Sodium perchlorate (NaClO4): IC50 was 1 μΜ; Sodium thiocyanate (NaSCN): IC50 was 14 μΜ; Sodium nitrate (NaNO3): IC50 was 250 μΜ; Sodium Tetrafluoroborate (NaBF4): IC50 was 0.75 μΜ.  Additionally, a library of 17020 compounds was screened for the identification of new human NIS inhibitors. The identification was based on the magnitude of changes in iodide uptake using Human Embryonic Kidney 293 (HEK293) cells, stably transfected with the hNIS. The same experiments and with similar results were also performed in rat thyroid derived cells (FRTL5), which endogenously express NIS. Compounds that inhibited iodide uptake in a time-dependent manner were considered to act through direct NIS inhibition. In contrast, those compounds that had a delayed effect on iodide uptake were thought to act through a sodium gradient disruption system resulting in indirect inhibition of iodide transport. Perchlorate was used as a positive control in these experiments and, as expected, it blocked iodide uptake immediately and totally throughout the experiment. Dysidenin was also used as a control and the IC50 value identified was 2 μΜ. All the compounds that were used for these experiments were small drug-like molecules that have not been detected in the environment and they were named as ITBs (Iodide Transport Blockers).

- Lecat-Guillet et al., 2008b With the same fully automated radioiodide uptake assay, as described above, new NIS inhibitors were also identified. The organotrifluoroborate (BF3−) was found to inhibit iodide uptake with an IC50 value of 0.4 μM using rat-derived thyroid cells (FRTL5). The biological activity is rationalized by the presence of the ion BF3− as a minimal binding motif for substrate recognition at the iodide binding site.

- Lindenthal et al., 2009 With the use of a patch-clamp technique an analysis of the NIS inhibitors identified by Lecat-Guillet et al., 2008 (named ITB-1 to ITB-10 for "Iodide Transport Blockers") was evaluated in Xenopus oocytes expressing NIS to further assess the inhibitory effect of those molecules specifically on NIS activity. Four of those molecules (ITB-3, ITB-9, ITB-5 and ITB-4) were identified as the most potent, non-competitive NIS inhibitors. The effects of dysidenin were also analyzed with the same technique, as it had been reported to be a specific inhibitor of NIS (Vroye et al., 1998). It was found that dysidenin (50 μM) induced a rapid and reversible inhibition of the iodide (about 40%) of induced current in mNIS-expressing oocytes, but did not evoke any currents in the absence of iodide, suggesting that this effect was due to the inhibition of NIS activity.

- Greer et al., 2002 In human studies, potassium perchlorate was used to predict inhibition of thyroidal iodide uptake by applying the RAIU method. Greer et al., tested body weight adjusted doses of potassium perchlorate and an assessment of RAIU uptake was performed on day 2 and day 14 of treatment and 24 h following treatment termination (on day 15). The NOEL value for inhibition of thyroidal uptake was 0.007 mg/kg-day, while the true NEL value was estimated to be 0.0052 and 0.0064 mg/kg-day. According to the dose-response inhibition of iodide uptake the maximum percentage of iodide inhibition at the doses of 0.0052 and 0.0064 mg/kg-day is 8.3-9.5%, which is physiologically insignificant for a person with dietary sufficient iodine intake.

- Wen et al., 2016 By using human MCF-7 cells, a breast adenocarcinoma cell line, which express inducible NIS in the presence of all-trans retinoic acid (ATRA) it has been shown that inhibition of sterol regulatory element-binding proteins (SREBP) maturation by treatment with 25-hydroxycholesterol (5 µM) for 48 hr reduced ATRA (1 µM)-induced mRNA concentration of NIS and decreased iodide uptake by approximately 20%. This study showed for the first time that the NIS gene and iodide uptake are regulated by SREBP in cultured human mammary epithelial cells.

- Arriagada et al. 2015 This study showed that 2 hr or 5 hr exposure to excess I- (100 μM) respectively in FRTL-5 cells and in ex-vivo rat thyroid gland (removed after single in vivo i.p. injection of 100 μg of I in 500 μL of distilled water, and analysis of 125I thyroid uptake), induced inhibition of I- uptake through the NIS (~ 30% uptake inhibition after 5 hr in vivo), a process known as the Wolff-Chaikoff effect, which was not associated with a decrease of NIS expression or a change in NIS localization. Incubation of FRTL-5 cells with excess I- for 2 hr increased hydrogen peroxide generation. Also incubation with hydrogen peroxide (100 μM) decreased NIS-mediated I- transport, effect that was reverted by ROS scavengers.

Uncertainties or Inconsistencies

?

The thyroid system is quite complex and therefore some inconsistent results have been produced by recent studies. For example, it has been observed in healthy volunteers that a 6-month exposure to perchlorate at doses up to 3 mg/d (low doses) had no effect on thyroid function, including inhibition of thyroid iodide uptake as well as serum levels of thyroid hormones, TSH, and Tg (Braverman et al., 2006). However, this study was limited by the small sample size and is obviously underpowered.

Quantitative Understanding of the Linkage

?


For this relationship there is not enough data linking quantitatively the inhibition of NIS with the amount of thyroidal uptake. The NIS inhibition is possible to be directly measured by using the fact that the simultaneous transport of 2 Na+ and 1 I- generates a current, which could be easily measured with electrophysiological methods (Eskandari et al., 1997) or with patch clamp techniques (Van Sande et al., 2003). However, the exact stoichiometry of the molecules that are transferred is not yet known, meaning that in some cases it cannot be detected. For example, perchlorate does not cause depolarization of the cellular membrane, as it is thought to be transferred in 1 to 1 stoichiometry with the Na+ (Van Sande et al., 2003). However, I- uptake can also be measured in vivo, as shown in rats i.p. injected with 100 μg of I in 500 μL of distilled water (known to cause an inhibition of NIS- mediated I- transport), followed by analysis of radioactive 125I thyroid uptake (Arriagada et al. 2015). Further studies are needed to support quantitative evaluation of this KER.

Evidence Supporting Taxonomic Applicability

?


Empirical evidence comes from in vitro works using rat follicular cells (Cianchetta et al., 2010; Waltz et al., 2010; Lecat-Guillet et al., 2007; 2008; Lecat-Guillet et al., 2008b), human in vitro cell models (Wen et al., 2016) and in vivo data (Arriagada et al. 2015), as well as Xenopus oocytes (Lindenthal et al., 2009) and Zebrafish (Thienpont et al., 2011).

References

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Arriagada AA, Albornoz E, Opazo MC, Becerra A, Vidal G, Fardella C, Michea L, Carrasco N, Simon F, Elorza AA, Bueno SM, Kalergis AM, Riedel CA. (2015). Excess iodide induces an acute inhibition of the sodium/iodide symporter in thyroid male rat cells by increasing reactive oxygen species. Endocrinology. Apr;156(4):1540-51.

Braverman LE, Pearce EN, He X, Pino S, Seeley M, Beck B, Magnani B, Blount BC, Firek A. (2006). Effects of six months of daily low-dose perchlorate exposure on thyroid function in healthy volunteers. J Clin Endocrinol Metab. 91:2721-2724.

Cianchetta S, di Bernardo J, Romeo G, Rhoden KJ (2010). Perchlorate transport and inhibition of the sodium iodide symporter measured with the yellow fluorescent protein variant YFP-H148Q/I152L. Toxicol Appl Pharmacol. 243:372-380.

Darrouzet E, Lindenthal S, Marcellin D, Pellequer JL, Pourcher T. (2014). The sodium/iodide symporter: state of the art of its molecular characterization. Biocim Biophys Acta. 1838:244-253.

De Groef B, Decallonne BR, Van der Geyten S, Darras VM, Bouillon R. (2006). Perchlorate versus other environmental sodium/iodide symporter inhibitors: potential thyroid-related health effects. Europ J Endocr. 155:17-25.

Eskandari S, Loo DD, Dai G, Levy O, Wright M, Carrasco N. (1997). Thyroid Na+/I- symporter: mechanism, stoichiometry, and specificity. J Biol Chem 272: 27230-27238.

Greer MA, Goodman G, Pleus RC, Greer SE. (2002). Health effects assessment for environmental perchlorate contamination: the dose response for inhibition of thyroidal radioiodine uptake in humans. Environm Health Persp. 110: 927-937.

Jones PA, Pendlington RU, Earl LK, Sharma RK, Barrat MD. (1996). In vitro investigations of the direct effects of complex anions on thyroidal iodide uptake: identification of novel inhibitors. Toxicol. In Vitro. 10: 149-160.

Kosugi S, Bhayana S, Dean HJ. (1999). A novel mutation in the sodium/iodide symporter gene in the largest family with iodide transport defect. J Clin Endocrinol Metab. 84: 3248-3253.

Kosugi S, Okamoto H, Tamada A, Sanchez-Franco F. (2002). A novel peculiar mutation in the sodium/iodide symporter gene in Spanish siblings with iodide transport defect. J Clin Endocrinol Metab. 87: 3830–3836.

Lecat-Guillet N, Merer G, Lopez R, Pourcher T, Rousseau B, Ambroise Y. (2008a). Small-molecule inhibitors of sodium iodide symporter function. Chembiochem 9:889–895.

Lecat-Guillet N, Ambroise Y. (2008b). Discovery of aryltrifluoroborates as potent sodium/iodide symporter (NIS) inhibitors. Chem Med Chem 3:1207–1209.

Lecat-Guillet N, Merer G, Lopez R, Pourcher T, Rousseau B, Ambroise Y. (2007). A 96-well automated radioiodide uptake assay for sodium/iodide symporter inhibitors. Assay Drug Dev Technol 5:535-540.

Lindenthal S, Lecat-Guillet N, Ondo-Mendez A, Ambroise Y, Rousseau B, Pourcher T. (2009). Characterization of small-molecule inhibitors of the sodium iodide symporter. J Endocrinol 200:357–365.

Matsuda A, Kosugi S. (1997). A homozygous missense mutation of the sodium/iodide symporter gene causing iodide transport defect. J Clin Endocrinol Metab. 82: 3966-3971.

Montanelli L, Agretti P, Marco G, Bagattini B, Ceccarelli C, Brozzi F, Lettiero T, Cerbone M, Vitti P, Salerno M, Pinchera A, Tonacchera M. (2009). Congenital hypothyroidism and late-onset goiter: identification and characterization of a novel mutation in the sodium/iodide symporter of the proband and family members. Thyroid 19: 1419-1425.

Pohlenz J, Rosenthal IM, Weiss RE, Jhiang SM, Burant C, Refetoff S. (1998). Congenital hypothyroidism due to mutations in the sodium/iodide symporter. Identification of a nonsense mutation producing a downstream cryptic 3′ splice site. J Clin Invest. 101:1028-1035.

Pohlenz J, Medeiros-Neto G, Gross JL, Silveiro SP, Knobel M, Refetoff S. (1997). Hypothyroidism in a Brazilian kindred due to iodide trapping defect caused by a homozygous mutation in the sodium/iodide symporter gene. Biochem Biophys Res Commun. 240: 488-491.

Portulano C, Paroder-Belenitsky M, Carrasco N. (2014). The Na+/I- symporter (NIS): Mechanism and medical impact. Endocr Rev. 35: 106-149.

Rhoden KJ, Cianchetta S, Stivani V, Portulano C, Galietta LJV, Romeo G. (2007). Cell-based imaging of sodium iodide symporter activity with the yellow fluorescent protein variant YFP-H148Q/I152L. Am. J. Physiol., 292, pp. C814–C823.

Schlosser PM. (2016). Revision of the affinity constant for perchlorate binding to the sodium-iodide symporter based on in vitro and human in vivo data. J Appl Toxicol. Dec;36(12):1531-1535.

Spitzweg C, Morris JC. (2010). Genetics and phenomics of hypothyroidism and goiter due to NIS mutations. Mol Cell Endocrinol. 322: 56-63.

Szinnai G, Kosugi S, Derrien C, Lucidarme N, David V, Czernichow P, Polak M. (2006). Extending the clinical heterogeneity of iodide transport defect (ITD): a novel mutation R124H of the sodium/iodide symporter gene and review of genotype-phenotype correlations in ITD. J Clin Endocrinol Metab. 91: 1199–1204.

Thienpont B, Tingaud-Sequeira A, Prats E, Barat, C., Babin P.J, Raldua D, (2011). Zebrafish eleutheroembryos provide a suitable vertebrate model for screening chemicals that impair thyroid hormone synthesis. Environ Sci Technol 45, 7525-7532.

Tonacchera M, Agretti P, de Marco G, Elisei R, Perri A, Ambrogini E, De Servi M, Ceccarelli C, Viacava P, Refetoff S, Panunzi C, Bitti ML, Vitti P, Chiovato L, Pinchera A. (2003). Congenital hypothyroidism due to a new deletion in the sodium/iodide symporter protein. Clin Endocrinol. 59: 500–506.

Tonacchera M, Pinchera A, Dimida A, Ferrarini E, Agretti P, Vitti P, Santini F, Crump K, Gibbs J. (2004). Relative potencies and additivity of perchlorate, thiocyanate, nitrate, and iodide on the inhibition of radioactive iodide uptake by the human sodium iodide symporter. Thyroid. 14: 1012-1019.

Van Sande J, Massart C, Beauwens R, Schoutens A, Costagliola S, Dumont JE, Wolff J. (2003). Anion selectivity by the sodium iodide symporter. Endocrinology. 144: 247-252.

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