Key Event Title
|Level of Biological Organization|
Key Event Components
Key Event Overview
AOPs Including This Key Event
|AOP Name||Role of event in AOP|
|HDAC inhibition leads to impeded craniofacial development||AdverseOutcome|
Key Event Description
In order for cartilage structures to form, chondrocytes need to secrete large amounts of collagen. One outcome of the disturbances of NCC migration and differentiation is the distortion and reduced size of facial cartilage structures.
How It Is Measured or Detected
The appearance of facial cartilage structures is readily visible in ventral views of zebrafish embryos at 5 days post fertilization. Such structures as the ceratohyal, Meckel’s cartilage, and the palatoquadrate are visible using fluorescent collagen reporter lines. Measurements of the angle formed by the ceratohyal provide a quantitative readout.