To the extent possible under law, AOP-Wiki has waived all copyright and related or neighboring rights to KE:1560
Key Event Title
|Level of Biological Organization|
Key Event Components
Key Event Overview
AOPs Including This Key Event
|Mus musculus||Mus musculus||Moderate||NCBI|
|Birth to < 1 month|
Key Event Description
Proper differentiation during embryonic development is regulated by the expression of genes at the right time and space. If key regulator genes are not expressed or wrongly expressed this leads to a different cell type.
How It Is Measured or Detected
Differentiation can be measured e.g. by in vitro hESC or iPSC based differentiation systems. Pre-requisite for this is a well characterized and homogenous cell population. Then it can be measured by the analysis of altered genes by gene set enrichment analysis (GEA) comparing control with potentially disturbed differentiation.
In the context of embryonic brain development, immunofluorescence on brain sections can be used with antibodies against neuronal differentiation markers such Tuj1, NeuN and betaIII-tubulin.
Domain of Applicability