API

Event: 1563

Key Event Title

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Decreased proximal tubular vectorial transport

Short name

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Decreased proximal tubular vectorial transport

Biological Context

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Level of Biological Organization
Tissue


Organ term

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Key Event Components

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Process Object Action

Key Event Overview


AOPs Including This Key Event

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AOP Name Role of event in AOP
Complex I inhibition leads to Fanconi syndrome KeyEvent

Stressors

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Taxonomic Applicability

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Life Stages

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Sex Applicability

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Key Event Description

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Vectorial transport across the monolayer is the primary function of proximal tubule cells in the nephron. The cells transport both water and solutes via active and secondary active transporters.


How It Is Measured or Detected

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When proximal tubule cells are grown on filter inserts, the volume and composition of the medium in the apical and basolateral compartments can be studied separately to determine variations in a vectorial transport.

Fluorescent dyes can also be used to measure transport across the monolayer either directly (transport of the dye itself) or indirectly (competition with the dye).

A differential measure of the rate of water transport can also be used in this format (Wilmes et al. 2014)


Domain of Applicability

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References

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Wilmes, A., L. Aschauer, A. Limonciel, W. Pfaller, and P. Jennings. 2014. “Evidence for a Role of Claudin 2 as a Proximal Tubular Stress Responsive Paracellular Water Channel.” Toxicology and Applied Pharmacology 279(2)