API

Event: 1595

Key Event Title

?

Oxidation/denatuation of membrane proteins

Short name

?

Oxidation/denatuation of membrane proteins

Biological Context

?

Level of Biological Organization
Cellular

Cell term

?


Organ term

?


Key Event Components

?

Process Object Action

Key Event Overview


AOPs Including This Key Event

?

AOP Name Role of event in AOP
ROS-mediated chemical phototoxicity KeyEvent

Stressors

?


Taxonomic Applicability

?

Term Scientific Term Evidence Link
human Homo sapiens High NCBI

Life Stages

?

Life stage Evidence
All life stages High

Sex Applicability

?

Term Evidence
Mixed High

Key Event Description

?


Reactive oxygen species yielded from photoactivated chemicals can cause cross-linking of proteins and oxidation of sulfydryl groups resulting in disulfide cross-links (Dalle Carbonare and Pathak, 1992).


How It Is Measured or Detected

?


As for in vitro systems, sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) and other gel electrophoresis methodologies can detect denaturation of proteins (Dalle Carbonare and Pathak, 1992).


Domain of Applicability

?


Chemicals: This KE applies to a wide range of chemicals.  The chemicals generate a reactive species, such as reactive oxygen species, following absorption of photon energy from light within the range of natural sunlight (290-700 nm) (ICH, 2014, Onoue and Tsuda, 2006).

Sex: This KE applies to both males and females. 

Life stages: The relevant life stages for this KE are all life stages after born.

Taxonomic: This KE mainly applies to human.


Evidence for Perturbation by Stressor



References

?


Dalle Carbonare M, Pathak MA. Skin photosensitizing agents and the role of reactive oxygen species in photoaging. J Photochem Photobiol B. 1992;14:105-24.

ICH. ICH Guideline S10 Guidance on Photosafety Evaluation of Pharmaceuticals.: International Council on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use; 2014.

Onoue S, Tsuda Y. Analytical studies on the prediction of photosensitive/phototoxic potential of pharmaceutical substances. Pharmaceutical research. 2006;23:156-64.