API
Event: 1613
Key Event Title
Short name
Decrease, DHT levelBiological Context
| Level of Biological Organization |
|---|
| Cellular |
Cell term
Organ term
Key Event Components
| Process | Object | Action |
|---|
Key Event Overview
AOPs Including This Key Event
| AOP Name | Role of event in AOP |
|---|---|
| Cyp17A1 inhibition leads to undescended testes in mammals | KeyEvent |
| 5α-reductase,female fish | KeyEvent |
| 5α-reductase inhibition leading to short AGD | KeyEvent |
| Decreased testosterone synthesis leading to short AGD | KeyEvent |
Stressors
Taxonomic Applicability
Life Stages
Sex Applicability
Key Event Description
Reduction in DHT synthesis leads to a reduction in DHT circulating levels. 12
How It Is Measured or Detected
DHT levels in a sample can be measured by (High Performance) Liquid Chromatography. After sample fractionation, DHT can be identify by comparison with internal standards spectrum. Quantification of DHT levels can be performed using hormones measurements kits (ELISA), instrumental techniques (LC-MS) or liquid scintillation spectrometry (after radiolabeling).3
Domain of Applicability
References
|
1 Miller Walter L. (1988) Molecular Biology of Steroid Hormone Synthesis. Endocrine Reviews, 9(3): 295-318.https://doi.org/10.1210/edrv-9-3-295
2 Miller W.L. and Auchus R.J. (2011) The Molecular Biology, Biochemistry, and Physiology of Human Steroidogenesis and Its Disorders. Endocrine Reviews, 32(1): 81-151.https://doi.org/10.1210/er.2010-0013
3 Shiraishi S., Lee P.W., Leung A., Goh V.H., Swerdloff R.S. and Wang C. (2008) Simultaneous measurement of serum testosterone and dihydrotestosterone by liquid chromatography-tandem mass spectrometry. Clinical chemistry, 54(11): 1855-63.https://doi.org/10.1373/clinchem.2008.103846 |