API

Event: 982

Key Event Title

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Suppression, Suppression in mixed lymphocyte reaction (MLR)

Short name

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Suppression, Suppression in mixed lymphocyte reaction (MLR)

Biological Context

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Level of Biological Organization
Cellular

Cell term

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Organ term

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Key Event Components

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Process Object Action

Key Event Overview


AOPs Including This Key Event

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Stressors

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Taxonomic Applicability

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Life Stages

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Sex Applicability

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Key Event Description

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FK506-FKBP complex interferes with NFAT nuclear localization to suppress the production of multiple classes of cytokines in T cells including L-2, which develops cytotoxic T cells as well as other actions. The suppressive effect of FK506 on mouse MLR, which has been thought to be the representative of IL-2 dependent T cell growth (Kino et al. 1987b). Main mode of actions for suppression of MLR with FK506 deems to result from the decreased production of cytokines including IL-2


How It Is Measured or Detected

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Suppression of cytotoxic T lymphocyte (CTL) activity can be measured by 51Cr release CTL assay. Suppression of MLR is also measurable by Incorporation of radioisotope (3H-thymidine).


Domain of Applicability

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FK-506 suppresses production of IL-2, IL-3, and IFN-γ in mouse MLR (Kino et al. 1987b). It also suppresses production of cytotoxic T lymphocytes (CTL) (Kino et al. 1987a).In human MLR, as well, FK-506 has been reported to suppress IL-2 production and expression of IL-2 receptors (Kino et al. 1987a).


References

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[1] Kino, T., Hatanaka, H., Miyata, S., Inamura, N., Nishiyama, M., Yajima, T., Goto, T., Okuhara, M., Kohsaka, M. and Aoki, H. (1987a). FK-506, a novel immunosuppressant isolated from a Streptomyces. II. Immunosuppressive effect of FK-506 in vitro. Journal of antibiotics. 40(9): 1256-1265.

[2] Kino, T., Hatanaka, H., Hashimoto, M., Nishiyama, M., Goto, T., Okuhara, M., Kohsaka, M., Aoki, H. and Imanaka, H. (1987b). FK-506, a novel immunosuppressant isolated from a Streptomyces. I. Fermentation, isolation, and physico-chemical and biological characteristics. Journal of antibiotics. 40(9): 1249-1255.