AOPs Including This Stressor
Events Including This Stressor
|Repression of Gbx2 expression|
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Zebrafish embryos were treated with chemicals from 14 hpf to 18 hpf, immediately before the advent of gbx2 expression in the telencephalon, and then gbx2 expression was examined in this brain region. In embryos where FGF signaling was inhibited by SU5402, gbx2 was downregulated in the telencephalon and MHB, but its expression in the OV was little affected (Z. Wang et al., 2018).
Characterization of Exposure
To clarify the critical stages of gbx2 regulation in the telencephalon, chemical treatment of zebrafish embryos started between 14 and 17 hpf and gbx2 expression was examined at 18 hpf. Alternatively, chemical treatment was started at 14 hpf and then embryos were washed between 15 and 18 hpf, cultured in the absence of chemicals, and gbx2 expression was examined at 18 hpf. Results showed SU5402 mediated repression of gbx2 expression in the telencephalon and MHB became less significant as the treatment start time was delayed from 14 hpf to 17 hpf, and gbx2 expression was gradually restored with earlier removal of the chemical, showing that FGF signaling is continuously required for gbx2 expression in the telencephalon (Z. Wang et al., 2018).
Wang, Z., Nakayama, Y., Tsuda, S., & Yamasu, K. (2018). The role of gastrulation brain homeobox 2 (gbx2) in the development of the ventral telencephalon in zebrafish embryos. Differentiation, 99(December 2017), 28–40. https://doi.org/10.1016/j.diff.2017.12.005