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Event: 1494

Key Event Title

The KE title should describe a discrete biological change that can be measured. It should generally define the biological object or process being measured and whether it is increased, decreased, or otherwise definably altered relative to a control state. For example “enzyme activity, decreased”, “hormone concentration, increased”, or “growth rate, decreased”, where the specific enzyme or hormone being measured is defined. More help

Leukocyte recruitment/activation

Short name
The KE short name should be a reasonable abbreviation of the KE title and is used in labelling this object throughout the AOP-Wiki. The short name should be less than 80 characters in length. More help
Leukocyte recruitment/activation

Biological Context

Structured terms, selected from a drop-down menu, are used to identify the level of biological organization for each KE. Note, KEs should be defined within a particular level of biological organization. Only KERs should be used to transition from one level of organization to another. Selection of the level of biological organization defines which structured terms will be available to select when defining the Event Components (below). More help

Cell term

Further information on Event Components and Biological Context may be viewed on the attached pdf.The biological context describes the location/biological environment in which the event takes place.  For molecular/cellular events this would include the cellular context (if known), organ context, and species/life stage/sex for which the event is relevant. For tissue/organ events cellular context is not applicable.  For individual/population events, the organ context is not applicable. More help

Organ term

Further information on Event Components and Biological Context may be viewed on the attached pdf.The biological context describes the location/biological environment in which the event takes place.  For molecular/cellular events this would include the cellular context (if known), organ context, and species/life stage/sex for which the event is relevant. For tissue/organ events cellular context is not applicable.  For individual/population events, the organ context is not applicable. More help

Key Event Components

Further information on Event Components and Biological Context may be viewed on the attached pdf.Because one of the aims of the AOP-KB is to facilitate de facto construction of AOP networks through the use of shared KE and KER elements, authors are also asked to define their KEs using a set of structured ontology terms (Event Components). In the absence of structured terms, the same KE can readily be defined using a number of synonymous titles (read by a computer as character strings). In order to make these synonymous KEs more machine-readable, KEs should also be defined by one or more “event components” consisting of a biological process, object, and action with each term originating from one of 22 biological ontologies (Ives, et al., 2017; See List). Biological process describes dynamics of the underlying biological system (e.g., receptor signalling). The biological object is the subject of the perturbation (e.g., a specific biological receptor that is activated or inhibited). Action represents the direction of perturbation of this system (generally increased or decreased; e.g., ‘decreased’ in the case of a receptor that is inhibited to indicate a decrease in the signalling by that receptor).Note that when editing Event Components, clicking an existing Event Component from the Suggestions menu will autopopulate these fields, along with their source ID and description. To clear any fields before submitting the event component, use the 'Clear process,' 'Clear object,' or 'Clear action' buttons. If a desired term does not exist, a new term request may be made via Term Requests. Event components may not be edited; to edit an event component, remove the existing event component and create a new one using the terms that you wish to add. More help
Process Object Action
cell activation involved in immune response leukocyte increased

Key Event Overview

AOPs Including This Key Event

All of the AOPs that are linked to this KE will automatically be listed in this subsection. This table can be particularly useful for derivation of AOP networks including the KE. Clicking on the name of the AOP will bring you to the individual page for that AOP. More help
AOP Name Role of event in AOP Point of Contact Author Status OECD Status
lysosomal uptake induced liver fibrosis KeyEvent Marina Kuburic (send email) Under development: Not open for comment. Do not cite EAGMST Under Review
Increased DNA damage leading to breast cancer KeyEvent Jessica Helm (send email) Under development: Not open for comment. Do not cite Under Development
RONS leading to breast cancer KeyEvent Jessica Helm (send email) Under development: Not open for comment. Do not cite Under Development

Stressors

This is a structured field used to identify specific agents (generally chemicals) that can trigger the KE. Stressors identified in this field will be linked to the KE in a machine-readable manner, such that, for example, a stressor search would identify this as an event the stressor can trigger. NOTE: intermediate or downstream KEs in one AOP may function as MIEs in other AOPs, meaning that stressor information may be added to the KE description, even if it is a downstream KE in the pathway currently under development.Information concerning the stressors that may trigger an MIE can be defined using a combination of structured and unstructured (free-text) fields. For example, structured fields may be used to indicate specific chemicals for which there is evidence of an interaction relevant to this MIE. By linking the KE description to a structured chemical name, it will be increasingly possible to link the MIE to other sources of chemical data and information, enhancing searchability and inter-operability among different data-sources and knowledgebases. The free-text section “Evidence for perturbation of this MIE by stressor” can be used both to identify the supporting evidence for specific stressors triggering the MIE as well as to define broad chemical categories or other properties that classify the stressors able to trigger the MIE for which specific structured terms may not exist. More help

Taxonomic Applicability

Latin or common names of a species or broader taxonomic grouping (e.g., class, order, family) can be selected from an ontology. In many cases, individual species identified in these structured fields will be those for which the strongest evidence used in constructing the AOP was available in relation to this KE. More help
Term Scientific Term Evidence Link
human Homo sapiens NCBI
Vertebrates Vertebrates NCBI

Life Stages

The structured ontology terms for life-stage are more comprehensive than those for taxa, but may still require further description/development and explanation in the free text section. More help
Life stage Evidence
All life stages

Sex Applicability

The authors must select from one of the following: Male, female, mixed, asexual, third gender, hermaphrodite, or unspecific. More help
Term Evidence
Unspecific

Key Event Description

A description of the biological state being observed or measured, the biological compartment in which it is measured, and its general role in the biology should be provided. For example, the biological state being measured could be the activity of an enzyme, the expression of a gene or abundance of an mRNA transcript, the concentration of a hormone or protein, neuronal activity, heart rate, etc. The biological compartment may be a particular cell type, tissue, organ, fluid (e.g., plasma, cerebrospinal fluid), etc. The role in the biology could describe the reaction that an enzyme catalyses and the role of that reaction within a given metabolic pathway; the protein that a gene or mRNA transcript codes for and the function of that protein; the function of a hormone in a given target tissue, physiological function of an organ, etc. Careful attention should be taken to avoid reference to other KEs, KERs or AOPs. Only describe this KE as a single isolated measurable event/state. This will ensure that the KE is modular and can be used by other AOPs, thereby facilitating construction of AOP networks. More help

The inflammatory response is the cornerstone of the body’s defense mechanism against bacterial and viral pathogens, as well as physical-, chemical- and environmental-mediated tissue and organ damage. Leucocyte recruitment at the site of pathogen evasion or sterile tissue injury is a critical adaptation for the preservation of tissue integrity. Neutrophils are the cell population that acutely responds to the alterations of inflammatory micro-environment. Neutrophil infiltration takes place within 6-8 hours from the initiation of the inflammatory process and is followed by the recruitment of other cell populations, like monocytes, lymphocytes, and eosinophils, which either promote or drive the resolution of inflammation. Leukocyte infiltration into sites of infection or sterile inflammation is a tightly regulated process that follows a sequence of adhesive events, termed as leukocyte adhesion cascade. One can broadly generalize that most leukocytes follow a similar multi-step cascade in the peripheral (non-lymphoid) vasculature with some exceptions. Accordingly, an updated adhesion cascade in postcapillary venules involves free-flowing leukocytes initial attachment or tethering and slow velocity rolling (step 1),stable adhesion (arrest) on endothelial cells (step 2), leukocyte flattening (step 3), and subsequent crawling on the vascular endothelium, transendothelial cell migration (TEM) between (paracellular route) or through (transcellular) the vascular endothelium (step 4), and uropod elongation to complete transmigration of postcapillary venules (step 5). The initial attachment and rolling steps are initiated by interactions of endothelial E- and P-selectins and their counterreceptors on leukocytes L-selectin (Leick et al., 2014).

Each of these steps is necessary for effective leukocyte recruitment; these steps are not phases of inflammation, but represent the sequence of events from the perspective of each leukocyte. At any given moment they all happen in parallel, involving different leukocytes in the same microvessels.

From the initial selectin-dependent leukocyte tethering to endothelial cells to the final migration of leukocytes into the sub-endothelium, this process depends on the interplay between leukocyte receptors and endothelial cell counter-receptors, as well as on the presence of endogenous inhibitors of leukocyte adhesion enabling the targeted recruitment of leukocytes to inflamed tissues.

To enable the infiltration of leukocytes at the site of inflammation, a series of alterations in endothelial cells and leukocytes takes place:

  • regulation of the expression of adhesion molecules in leukocytes
  • increased secretion of chemokines by endothelial cells
  • increased expression of adhesion molecules in the luminal surface of endothelial cells

(Kourtzelis and Mitroulis, 2015) (Robbins and Cotran: Pathologic Basis of Disease 2010).

After recruitment, activation includes phenotype modification with morphologic alterations, changes in marker proteins (MHC, adhesion molecules, co-stimulatory signal), expression of mediators, enzymes, and pro-inflammatory proteins/lipids. Recruited monocytes recruited mature into macrophages with phagocytic activity and elaboration of a myriad of mediators of inflammation. The macrophage can replicate within tissues or die, including by apoptosis.

How It Is Measured or Detected

One of the primary considerations in evaluating AOPs is the relevance and reliability of the methods with which the KEs can be measured. The aim of this section of the KE description is not to provide detailed protocols, but rather to capture, in a sentence or two, per method, the type(s) of measurements that can be employed to evaluate the KE and the relative level of scientific confidence in those measurements. Methods that can be used to detect or measure the biological state represented in the KE should be briefly described and/or cited. These can range from citation of specific validated test guidelines, citation of specific methods published in the peer reviewed literature, or outlines of a general protocol or approach (e.g., a protein may be measured by ELISA).Key considerations regarding scientific confidence in the measurement approach include whether the assay is fit for purpose, whether it provides a direct or indirect measure of the biological state in question, whether it is repeatable and reproducible, and the extent to which it is accepted in the scientific and/or regulatory community. Information can be obtained from the OECD Test Guidelines website and the EURL ECVAM Database Service on Alternative Methods to Animal Experimentation (DB-ALM). ?

in vivo imaging:

  • Flow cytometry (FC/FACS),
  • immunhistochemistry
  • two photon-intravital microscopy (TP-IVM) (van Grinsven et al., 2017)
  • Spinning Disk Confocal Microscopy-IVM (Jenne et al., 2011)
  • Histology, increased cell numbers and altered composition

In vitro

  • transwell Migration Assay (Justus et al., 2014)
  • T-Lymphocyte & Innate Immune Cell Activation Assays
  • Leukocyte Surface Markers (Monoclonal Antibodies to Leukocyte Surface Markers)
  • Markers of leukocyte activation – protease release, ROS/RNS, NADPH oxidase (NOX), defense response - expression of anti-oxidants.
  • organs-on-a-chip (Bnam et al., 2016; Ribas et al., 2017; Wufuer et al. 2016)

REFERENCES:

Benam KH, Villenave R, Lucchesi C, Varone 1, Hubeau C, Lee HH, Alves SE, Salmon M, Ferrante TC, Weaver JC, Bahinski A, Hamilton GA, Ingber DE., Small airway-on-a-chip enables analysis of human lung inflammation and drug responses in vitro, Nat Methods. 2016 Feb;13(2):151-7.

Ribas, J., Zhang, Y. S., Pitrez, P. R., Leijten, J., Miscuglio, M., Rouwkema, J., Dokmeci, M. R., Nissan, X., Ferreira, L. and Khademhosseini, A. (2017), Organ-On-A-Chip: Biomechanical Strain Exacerbates Inflammation on a Progeria-on-a-Chip Model doi:10.1002/smll.201770087

Wufuer M, Lee G, Hur W, Jeon B, Kim BJ, Choi  TH, Lee SH, Skin-on-a-chip model simulating inflammation, edema and drug-based treatment,  Nature Scientific Reports 6, Article number: 37471 (2016) doi:10.1038/srep37471

Domain of Applicability

This free text section should be used to elaborate on the scientific basis for the indicated domains of applicability and the WoE calls (if provided). While structured terms may be selected to define the taxonomic, life stage and sex applicability (see structured applicability terms, above) of the KE, the structured terms may not adequately reflect or capture the overall biological applicability domain (particularly with regard to taxa). Likewise, the structured terms do not provide an explanation or rationale for the selection. The free-text section on evidence for taxonomic, life stage, and sex applicability can be used to elaborate on why the specific structured terms were selected, and provide supporting references and background information.  More help

References

List of the literature that was cited for this KE description. Ideally, the list of references, should conform, to the extent possible, with the OECD Style Guide (https://www.oecd.org/about/publishing/OECD-Style-Guide-Third-Edition.pdf) (OECD, 2015). More help

Kourtzelis I and Mitroulis I, Encyclopedia of Inflammatory Diseases, Leukocyte Recruitment, pp 1-9, Compendium of Inflammatory Diseases, Editors: Michael J. Parnham , Springer Basel, 2015, DOI 10.1007/978-3-0348-0620-6_177-1

Kumar, V.; Abbas, AK.; Fausto, N.; Aster, J. Robbins and Cotran: Pathologic Basis of Disease. 8. Elsevier; Philadelphia: 2010.

Leick M, Azcutia V, Newton G, Luscinskas FW., Leukocyte recruitment in inflammation: basic concepts and new mechanistic insights based on new models and microscopic imaging technologies, Cell Tissue Res. 2014 Mar;355(3):647-56

Nourshargh S, Alon R., Leukocyte migration into inflamed tissues., Immunity. 2014 Nov 20;41(5):694-707