API
Event: 1610
Key Event Title
Short name
Reduction, DHEABiological Context
| Level of Biological Organization |
|---|
| Cellular |
Cell term
Organ term
Key Event Components
| Process | Object | Action |
|---|
Key Event Overview
AOPs Including This Key Event
| AOP Name | Role of event in AOP |
|---|---|
| Cyp17A1 inhibition leads to undescended testes in mammals | KeyEvent |
Stressors
Taxonomic Applicability
Life Stages
Sex Applicability
Key Event Description
17-OH-pregnenolone is the direct precursor of dehydroepiandrosterone (DHEA), a reduction in its synthesis results in a decrease in DHEA level. DHEA is defined as an obligatory intermediate in sex steroid biosynthesis being the precursor of steroid hormones like testosterone and estradiol.12
How It Is Measured or Detected
17-OH-pregnenolone and DHEA can be fractionated using High Performance Liquid Chromatography. After separation, pregnenolone and DHEA levels can be quantify using immunoassay such as ELISA or Radio Immuno Assay (RIA). For both steroids, LC-MS/MS is also an option.
Domain of Applicability
References
1 Miller Walter L. (1988) Molecular Biology of Steroid Hormone Synthesis. Endocrine Reviews, 9(3): 295-318. https://doi.org/10.1210/edrv-9-3-295
2 Miller W.L. and Auchus R.J. (2011) The Molecular Biology, Biochemistry, and Physiology of Human Steroidogenesis and Its Disorders. Endocrine Reviews, 32(1): 81-151.https://doi.org/10.1210/er.2010-0013