API

Event: 1610

Key Event Title

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Reduction, 17-OH-pregnenolone conversion in DHEA

Short name

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Reduction, DHEA

Biological Context

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Level of Biological Organization
Cellular

Cell term

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Organ term

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Key Event Components

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Process Object Action

Key Event Overview


AOPs Including This Key Event

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AOP Name Role of event in AOP
Cyp17A1 inhibition leads to undescended testes in mammals KeyEvent

Stressors

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Taxonomic Applicability

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Life Stages

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Sex Applicability

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Key Event Description

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17-OH-pregnenolone is the direct precursor of dehydroepiandrosterone (DHEA), a reduction in its synthesis results in a decrease in DHEA level. DHEA is defined as an obligatory intermediate in sex steroid biosynthesis being the precursor of steroid hormones like testosterone and estradiol.12


How It Is Measured or Detected

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17-OH-pregnenolone and DHEA can be fractionated using High Performance Liquid Chromatography. After separation, pregnenolone and DHEA levels can be quantify using immunoassay such as ELISA or Radio Immuno Assay (RIA). For both steroids, LC-MS/MS is also an option.


Domain of Applicability

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References

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1 Miller Walter L. (1988) Molecular Biology of Steroid Hormone Synthesis. Endocrine Reviews, 9(3): 295-318. https://doi.org/10.1210/edrv-9-3-295 

2 Miller W.L. and Auchus R.J. (2011) The Molecular Biology, Biochemistry, and Physiology of Human Steroidogenesis and Its Disorders. Endocrine Reviews, 32(1): 81-151.https://doi.org/10.1210/er.2010-0013