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Event: 41
Key Event Title
Inhibition, Bile Salt Export Pump (ABCB11)
Short name
Biological Context
Level of Biological Organization |
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Molecular |
Cell term
Cell term |
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eukaryotic cell |
Organ term
Key Event Components
Process | Object | Action |
---|---|---|
transport | bile salt export pump | decreased |
Key Event Overview
AOPs Including This Key Event
AOP Name | Role of event in AOP | Point of Contact | Author Status | OECD Status |
---|---|---|---|---|
Cholestatic Liver Injury induced by Inhibition of the Bile Salt Export Pump (ABCB11) | MolecularInitiatingEvent | Mathieu Vinken (send email) | Under development: Not open for comment. Do not cite | Under Development |
Taxonomic Applicability
Term | Scientific Term | Evidence | Link |
---|---|---|---|
humans | Homo sapiens | High | NCBI |
Life Stages
Sex Applicability
Key Event Description
The BSEP transporter protein is a prominent adenosine triphosphate-binding cassette transporter located at the canalicular pole of the hepatocyte membrane, which conveys bile acids from the hepatocyte cytosol into the bile canaliculi (Kis et al., 2012). BSEP can be directly cis-inhibited in a competitive way by several cholestasis-inducing drugs, including cyclosporine A, rifampicin, erythromycin estolate, 17α-ethinyl estradiol, bosentan, troglitazone and glibenclamide (Dawson et al., 2011; Kis et al., 2012; Morgan et al., 2010; Padda et al., 2011; Wagner et al., 2009; Zollner and Trauner, 2006 and 2008). As a result of BSEP inhibition, toxic bile acids, such as glycochenodeoxycholic acid, accumulate into the cytosol of the hepatocytes or bile canaliculi into so-called bile plugs (Kuntz and Kuntz, 2008).
How It Is Measured or Detected
A number of in vitro assays for measuring BSEP inhibition have been developed and successfully applied to demonstrate the cholestasis-inducing potential of drugs (Dawson et al., 2011; Kis et al., 2012; Morgan et al., 2010).
Domain of Applicability
References
Dawson, S., Stahl, S., Paul, N., Barber, J., Kenna, J.G. (2011). In vitro inhibition of the bile salt export pump correlates with risk of cholestatic drug-induced liver injury in humans. Drug Metab. Dispos. 40, 130-138.
Kis, E., Ioja, E., Rajnai, Z., Jani, M., Méhn, D., Herédi-Szabó, K., Krajcsi, P. (2012). BSEP inhibition: in vitro screens to assess cholestatic potential of drugs. Toxicol. In Vitro 26, 1294-1299.
Kuntz, E., Kuntz, H.D. (2008). Cholestasis. In Hepatology: Textbook and Atlas : History, Morphology, Biochemistry, Diagnostics, Clinic, Therapy 3rd edition (E. Kuntz E., H.-D. Kuntz Eds.), pp. 235-250. Springer, Heidelberg.
Morgan, R.E., Trauner, M., van Staden, C.J., Lee, P.H., Ramachandran, B., Eschenberg, M., Afshari, C.A., Qualls, C.W. Jr., Lightfoot-Dunn, R., Hamadeh, H.K. (2010). Interference with bile salt export pump function is a susceptibility factor for human liver injury in drug development. Toxicol. Sci. 118, 485-500.
Padda, M.S., Sanchez, M., Akhtar, A.J., Boyer, J.L. (2011). Drug-induced cholestasis. Hepatology 53, 1377-1387.
Wagner, M., Zollner, G., Trauner, M. (2009). New molecular insights into the mechanisms of cholestasis. J. Hepatol. 51, 565-580.
Zollner, G., Trauner, M. (2006). Molecular mechanisms of cholestasis. Wien. Med. Wochenschr. 156, 380-385.
Zollner, G., Trauner, M. (2008). Mechanisms of cholestasis. Clin. Liver Dis. 12, 1-26.