API

Event: 1562

Key Event Title

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Decreased Na/K ATPase activity

Short name

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Decreased Na/K ATPase activity

Biological Context

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Level of Biological Organization
Cellular

Cell term

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Organ term

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Key Event Components

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Process Object Action

Key Event Overview


AOPs Including This Key Event

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AOP Name Role of event in AOP
Complex I inhibition leads to Fanconi syndrome KeyEvent

Stressors

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Taxonomic Applicability

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Life Stages

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Sex Applicability

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Key Event Description

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The sodium/potassium (Na/K) ATPase is an active pump that consumes ATP to transport 3 sodium ions (Na + ) out of the cell against its electrochemical gradient, in exchange for 2 potassium ions (K + ). A decrease in Na/K activity can come from a decrease in ATP available to perform this reaction or direct blocking of the pump.


How It Is Measured or Detected

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Na/K ATPase activity can be measured in cell lysates preserving enzymatic activity, and corresponds to the difference between total ATPase activity and ouabain-sensitive ATPase activity. The measurement can use the leftover ATP or inorganic phosphate produced during ATP hydrolysis as its primary substrate, followed by coupling to colorimetric or fluorimetric dye (Baginski, Foa, and Zak 1967; Nowak 2002) (NovusBio kit 601-0120).


Domain of Applicability

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References

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Baginski, E. S., P. P. Foa, and B. Zak. 1967. “Determination of Phosphate: Study of Labile Organic Phosphate Interference.” Clinica Chimica Acta 15(1):155–58. Retrieved December 6, 2017

Nowak, Grazyna. 2002. “Protein Kinase C-Alpha and ERK1/2 Mediate Mitochondrial Dysfunction, Decreases in Active Na+ Transport, and Cisplatin-Induced Apoptosis in Renal Cells.” The Journal of Biological Chemistry 277(45):43377–88. Retrieved December 5, 2017 (http://www.ncbi.nlm.nih.gov/pubmed/12218054).