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Key Event Title
Decreased Na/K ATPase activity
|Level of Biological Organization|
Key Event Components
Key Event Overview
AOPs Including This Key Event
|AOP Name||Role of event in AOP||Point of Contact||Author Status||OECD Status|
|Complex I inhibition leads to Fanconi syndrome||KeyEvent||Marvin Martens (send email)||Under development: Not open for comment. Do not cite|
|Uncoupling of OXPHOS leading to growth inhibition 4||KeyEvent||You Song (send email)||Under development: Not open for comment. Do not cite||Under Development|
Key Event Description
The sodium/potassium (Na/K) ATPase is an active pump that consumes ATP to transport 3 sodium ions (Na + ) out of the cell against its electrochemical gradient, in exchange for 2 potassium ions (K + ). A decrease in Na/K activity can come from a decrease in ATP available to perform this reaction or direct blocking of the pump.
How It Is Measured or Detected
Na/K ATPase activity can be measured in cell lysates preserving enzymatic activity, and corresponds to the difference between total ATPase activity and ouabain-sensitive ATPase activity. The measurement can use the leftover ATP or inorganic phosphate produced during ATP hydrolysis as its primary substrate, followed by coupling to colorimetric or fluorimetric dye (Baginski, Foa, and Zak 1967; Nowak 2002) (NovusBio kit 601-0120).
Domain of Applicability
Baginski, E. S., P. P. Foa, and B. Zak. 1967. “Determination of Phosphate: Study of Labile Organic Phosphate Interference.” Clinica Chimica Acta 15(1):155–58. Retrieved December 6, 2017
Nowak, Grazyna. 2002. “Protein Kinase C-Alpha and ERK1/2 Mediate Mitochondrial Dysfunction, Decreases in Active Na+ Transport, and Cisplatin-Induced Apoptosis in Renal Cells.” The Journal of Biological Chemistry 277(45):43377–88. Retrieved December 5, 2017 (http://www.ncbi.nlm.nih.gov/pubmed/12218054).