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Relationship: 2212

Title

A descriptive phrase which clearly defines the two KEs being considered and the sequential relationship between them (i.e., which is upstream, and which is downstream). More help

Reduced, Swimming performance leads to Increased Mortality

Upstream event
The causing Key Event (KE) in a Key Event Relationship (KER). More help
Reduced, Swimming performance
Downstream event
The responding Key Event (KE) in a Key Event Relationship (KER). More help
Increased Mortality

Key Event Relationship Overview

The utility of AOPs for regulatory application is defined, to a large extent, by the confidence and precision with which they facilitate extrapolation of data measured at low levels of biological organisation to predicted outcomes at higher levels of organisation and the extent to which they can link biological effect measurements to their specific causes.Within the AOP framework, the predictive relationships that facilitate extrapolation are represented by the KERs. Consequently, the overall WoE for an AOP is a reflection in part, of the level of confidence in the underlying series of KERs it encompasses. Therefore, describing the KERs in an AOP involves assembling and organising the types of information and evidence that defines the scientific basis for inferring the probable change in, or state of, a downstream KE from the known or measured state of an upstream KE. More help

AOPs Referencing Relationship

AOP Name Adjacency Weight of Evidence Quantitative Understanding Point of Contact Author Status OECD Status
Deiodinase 2 inhibition leading to increased mortality via reduced posterior swim bladder inflation adjacent Moderate Low Dries Knapen (send email) Under Development: Contributions and Comments Welcome WPHA/WNT Endorsed
Deiodinase 2 inhibition leading to increased mortality via reduced anterior swim bladder inflation adjacent Moderate Low Dries Knapen (send email) Under Development: Contributions and Comments Welcome WPHA/WNT Endorsed
Deiodinase 1 inhibition leading to increased mortality via reduced posterior swim bladder inflation adjacent Moderate Low Dries Knapen (send email) Under Development: Contributions and Comments Welcome WPHA/WNT Endorsed
Deiodinase 1 inhibition leading to increased mortality via reduced anterior swim bladder inflation adjacent Moderate Low Dries Knapen (send email) Under Development: Contributions and Comments Welcome WPHA/WNT Endorsed
Thyroperoxidase inhibition leading to increased mortality via reduced anterior swim bladder inflation adjacent Moderate Low Dries Knapen (send email) Under Development: Contributions and Comments Welcome WPHA/WNT Endorsed

Taxonomic Applicability

Latin or common names of a species or broader taxonomic grouping (e.g., class, order, family) that help to define the biological applicability domain of the KER.In general, this will be dictated by the more restrictive of the two KEs being linked together by the KER.  More help
Term Scientific Term Evidence Link
zebrafish Danio rerio Moderate NCBI
fathead minnow Pimephales promelas Moderate NCBI

Sex Applicability

An indication of the the relevant sex for this KER. More help
Sex Evidence
Unspecific Moderate

Life Stage Applicability

An indication of the the relevant life stage(s) for this KER.  More help
Term Evidence
Adult Moderate
Juvenile Moderate
Larvae Moderate

Key Event Relationship Description

Provides a concise overview of the information given below as well as addressing details that aren’t inherent in the description of the KEs themselves. More help

Reduced swimming performance is likely to affect essential endpoints such as predator avoidance, feeding behaviour and reproduction in taxa that rely on swimming to support these vital behaviours. These parameters are biologicaly plausible to affect survival, especially in a non-laboratory environment where food is scarce and predators are abundant.

Evidence Collection Strategy

Include a description of the approach for identification and assembly of the evidence base for the KER. For evidence identification, include, for example, a description of the sources and dates of information consulted including expert knowledge, databases searched and associated search terms/strings.  Include also a description of study screening criteria and methodology, study quality assessment considerations, the data extraction strategy and links to any repositories/databases of relevant references.Tabular summaries and links to relevant supporting documentation are encouraged, wherever possible. More help

Evidence Supporting this KER

Addresses the scientific evidence supporting KERs in an AOP setting the stage for overall assessment of the AOP. More help

A direct relationship between reduced swimming performance and reduced survival is difficult to establish. There is however a lot of indirect evidence linking reduced swim bladder inflation to reduced survival (https://aopwiki.org/relationships/2213), which can be plausibly assumed to be related to reduced swimming performance.

For example, all zebrafish larvae that failed to inflate the posterior chamber after exposure to 2 mg/L iopanoic acid (IOP), died by the age of 9 dpf (Stinckens et al., 2020). Since larvae from the same group that were able to inflate the posterior chamber survived and the test was performed in the laboratory in optimal conditions, it is plausible to assume that the cause of death was the inability to swim and find food due to the failure to inflate the posterior swim bladder chamber.

Biological Plausibility
Addresses the biological rationale for a connection between KEupstream and KEdownstream.  This field can also incorporate additional mechanistic details that help inform the relationship between KEs, this is useful when it is not practical/pragmatic to represent these details as separate KEs due to the difficulty or relative infrequency with which it is likely to be measured.   More help

Reduced swimming performance is likely to affect essential endpoints such as predator avoidance, feeding behaviour and reproduction. These parameters are biologicaly plausible to affect survival, especially in a non-laboratory environment where food is scarce and predators are abundant.

Uncertainties and Inconsistencies
Addresses inconsistencies or uncertainties in the relationship including the identification of experimental details that may explain apparent deviations from the expected patterns of concordance. More help

A direct relationship between reduced swimming performance and reduced survival is difficult to establish in a laboratory environment where food is abundant and there are no predators.

Known modulating factors

This table captures specific information on the MF, its properties, how it affects the KER and respective references.1.) What is the modulating factor? Name the factor for which solid evidence exists that it influences this KER. Examples: age, sex, genotype, diet 2.) Details of this modulating factor. Specify which features of this MF are relevant for this KER. Examples: a specific age range or a specific biological age (defined by...); a specific gene mutation or variant, a specific nutrient (deficit or surplus); a sex-specific homone; a certain threshold value (e.g. serum levels of a chemical above...) 3.) Description of how this modulating factor affects this KER. Describe the provable modification of the KER (also quantitatively, if known). Examples: increase or decrease of the magnitude of effect (by a factor of...); change of the time-course of the effect (onset delay by...); alteration of the probability of the effect; increase or decrease of the sensitivity of the downstream effect (by a factor of...) 4.) Provision of supporting scientific evidence for an effect of this MF on this KER. Give a list of references.  More help
Response-response Relationship
Provides sources of data that define the response-response relationships between the KEs.  More help
Time-scale
Information regarding the approximate time-scale of the changes in KEdownstream relative to changes in KEupstream (i.e., do effects on KEdownstream lag those on KEupstream by seconds, minutes, hours, or days?). More help

Reduced swimming performance is not expected to immediately lead to mortality. Depending on the extent of the reduction in swimming performance and depending on the cause of death (e.g., starvation due to the inability to find food, being caught by a predator) the lag time may vary.

As an example, Stinckens et al. (2020) found that zebrafish larvae that failed to inflate the swim bladder at 5 dpf and did not manage to inflate it during the days afterwards died by the age of 9 dpf. Since zebrafish initiate exogenous feeding around 5 dpf when the yolk is almost completely depleted, there was a lag period of around 4 days after which reduced feeding resulted in mortality. Obviously, in a laboratory setup there is no increased risk of being caught by a predator.

Known Feedforward/Feedback loops influencing this KER
Define whether there are known positive or negative feedback mechanisms involved and what is understood about their time-course and homeostatic limits. More help

Domain of Applicability

A free-text section of the KER description that the developers can use to explain their rationale for the taxonomic, life stage, or sex applicability structured terms. More help

Importance of swimming performance on survival is generally applicable to all hatched fish across life stages and sexes and to other taxa that rely on swimming to support vital behaviours.

References

List of the literature that was cited for this KER description. More help

Stinckens, E., Vergauwen, L., Blackwell, B.R., Anldey, G.T., Villeneuve, D.L., Knapen, D., 2020. Effect of Thyroperoxidase and Deiodinase Inhibition on Anterior Swim Bladder Inflation in the Zebrafish. Environmental Science & Technology 54, 6213-6223.