This AOP is licensed under a Creative Commons Attribution 4.0 International License.
Increased reactive oxygen species production leading to population decline via mitochondrial dysfunction
Point of Contact
- Knut Erik Tollefsen
- You Song
- Li Xie
|Author status||OECD status||OECD project||SAAOP status|
|Under development: Not open for comment. Do not cite|
This AOP was last modified on June 04, 2021 17:25
|Decrease, Mitochondrial membrane potential||April 30, 2020 12:41|
|Decrease, Oxidative phosphorylation||December 20, 2018 10:16|
|Decrease, Mitochondrial ATP production||September 16, 2017 10:14|
|Decrease, Leaf development||October 10, 2017 07:54|
|Decrease, Reproduction||April 11, 2021 17:38|
|Decrease, Population trajectory||September 03, 2021 11:24|
|Increase, Reactive Oxygen Species production||April 11, 2021 18:03|
|Increase, ROS production leads to Decrease, MMP||April 30, 2020 12:43|
|Decrease, MMP leads to Decrease, OXPHOS||April 30, 2020 12:44|
|Decrease, OXPHOS leads to Decrease, Mitochondrial ATP production||October 10, 2017 08:00|
|Decrease, Leaf development leads to Decrease, Reproduction||April 11, 2021 08:26|
|Decrease, Reproduction leads to Decrease, Population trajectory||April 11, 2021 08:26|
|Ionizing Radiation||May 07, 2019 12:12|
|Gamma radiation||April 15, 2017 16:04|
|Ultraviolet B radiation||April 15, 2017 16:04|
Ionising and non-ionizing radiation may excite and/or ionize intracellular water molecules to increase production of reactive oxygen species (ROS). Such excessive ROS production can trigger a reduction in the mitochondrial membrane potential, reduce oxidative phosphorylation and reduce ATP production. Reduction in the ATP pool may consequently reduce available energy to support development (size and weight) and/or reproduction (number of organisms and/or leaves) to reduce the overall population.
Summary of the AOP
Molecular Initiating Events (MIE)
Key Events (KE)
Adverse Outcomes (AO)
|Sequence||Type||Event ID||Title||Short name|
|MIE||257||Increase, Reactive Oxygen Species production||Increase, ROS production|
|KE||1770||Decrease, Mitochondrial membrane potential||Decrease, MMP|
|KE||1477||Decrease, Oxidative phosphorylation||Decrease, OXPHOS|
|KE||40||Decrease, Mitochondrial ATP production||Decrease, Mitochondrial ATP production|
|KE||1479||Decrease, Leaf development||Decrease, Leaf development|
Relationships Between Two Key Events (Including MIEs and AOs)
|Increase, ROS production leads to Decrease, MMP||adjacent||High||Moderate|
|Decrease, MMP leads to Decrease, OXPHOS||adjacent||Moderate||Moderate|
|Decrease, OXPHOS leads to Decrease, Mitochondrial ATP production||adjacent||High||High|
|Decrease, Leaf development leads to Decrease, Reproduction||adjacent||Moderate||Moderate|
|Decrease, Reproduction leads to Decrease, Population trajectory||adjacent||High||High|
Life Stage Applicability
|Adult, reproductively mature||High|
|Lemna minor||Lemna minor||High||NCBI|
Overall Assessment of the AOP
The evidence for the MIE, KE and AO were considered Moderate to High for all Key Events and Key Event Relationships. The overall assessment of the AOP were considered moderate.
Domain of Applicability
Taxonomic: all primary producers
Life stage: all stages
Sex: both genders (dioecious plants) and not relevant (hermaphrodites)
Stressors: Ionizing radiation, Ultraviolet B radiation (UVB)
Essentiality of the Key Events
The essentiality of all key events was considered as Moderate to High. Essentiality evaluations were mainly based on specifically designed studies demonstrating the expected effect pattern predicted by the AOP to occur after exposure to Cobalt-60 external radiation and Ultraviolet B (UVB) radiation.
Overall confidence in the AOP:
Quantitative data were generated in studies with the freshwater macrophyte Lemna minor exposed to external gamma radiation from a Cobalt-60 source (Ionising radiation) and artifical ultraviolet B radiation (UVB). The quantitative understanding of the AOP was therefore considered to be Moderate for these species.
Considerations for Potential Applications of the AOP (optional)
Xie, L., Solhaug, K. A., Song, Y., Brede, D. A., Lind, O. C., Salbu, B., & Tollefsen, K. E. (2019). Modes of action and adverse effects of gamma radiation in an aquatic macrophyte Lemna minor. Science of the Total Environment, 680, 23-34.
Xie, L., Solhaug, K. A., Song, Y., Johnsen, B., Olsen, J. E., & Tollefsen, K. E. (2020). Effects of artificial ultraviolet B radiation on the macrophyte Lemna minor: a conceptual study for toxicity pathway characterization. Planta, 252(5), 1-18.