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Key Event Title
Reduction, Vitellogenin synthesis in liver
Key Event Components
Key Event Overview
AOPs Including This Key Event
|AOP Name||Role of event in AOP||Point of Contact||Author Status||OECD Status|
|Aromatase inhibition leading to reproductive dysfunction||KeyEvent||Dan Villeneuve (send email)||Open for citation & comment||WPHA/WNT Endorsed|
|Androgen receptor agonism leading to reproductive dysfunction||KeyEvent||Dan Villeneuve (send email)||Open for citation & comment||WPHA/WNT Endorsed|
|Estrogen receptor antagonism leading to reproductive dysfunction||KeyEvent||Dan Villeneuve (send email)||Open for citation & comment||EAGMST Under Review|
|Prolyl hydroxylase inhibition||KeyEvent||Dalma Martinovic-Weigelt (send email)||Under Development: Contributions and Comments Welcome|
|Unknown MIE leading to reprodl||KeyEvent||Dalma Martinovic-Weigelt (send email)||Under Development: Contributions and Comments Welcome|
|AHR mediated epigenetic reproductive failure||KeyEvent||Jon Doering (send email)||Under development: Not open for comment. Do not cite|
|TPO inhibition and impaired fertility||KeyEvent||June-Woo Park (send email)||Open for comment. Do not cite||Under Development|
|Adult, reproductively mature||High|
Key Event Description
Vitellogenin is an egg yolk precursor protein synthesized by hepatocytes of oviparous vertebrates. In vertebrates, transcription of vitellogenin genes is predominantly regulated by estrogens via their action on nuclear estrogen receptors. During vitellogenic periods of the reproductive cycle, when circulating estrogen concentrations are high, vitellogenin transcription and synthesis are typically orders of magnitude greater than during non-reproductive conditions.
How It Is Measured or Detected
Relative abundance of vitellogenin transcripts or protein can be readily measured in liver tissue (e.g., (Biales et al. 2007)) or whole body (H Holbech et al. 2001) from organisms exposed in vivo, or in liver slices (e.g., (Schmieder et al. 2000) or hepatocytes (e.g., (Navas and Segner 2006) exposed in vitro, using real-time quantitative polymerase chain reaction (PCR; transcripts) or enzyme linked immunosorbent assay (ELISA; protein).
Domain of Applicability
Oviparous vertebrates. Although vitellogenin is conserved among oviparous vertebrates and many invertebrates, liver is not a relevant tissue for the production of vitellogenin in invertebrates (Wahli 1988)
- Biales AD, Bencic DC, Lazorchak JL, Lattier DL. 2007. A quantitative real-time polymerase chain reaction method for the analysis of vitellogenin transcripts in model and nonmodel fish species. Environ Toxicol Chem 26(12): 2679-2686.
- Navas JM, Segner H. 2006. Vitellogenin synthesis in primary cultures of fish liver cells as endpoint for in vitro screening of the (anti)estrogenic activity of chemical substances. Aquat Toxicol 80(1): 1-22.
- Schmieder P, Tapper M, Linnum A, Denny J, Kolanczyk R, Johnson R. 2000. Optimization of a precision-cut trout liver tissue slice assay as a screen for vitellogenin induction: comparison of slice incubation techniques. Aquat Toxicol 49(4): 251-268.
- Wahli W. 1988. Evolution and expression of vitellogenin genes. Trends in Genetics. 4:227-232.
H Holbech, L Andersen, G I Petersen, B Korsgaard, K L Pedersen, P Bjerregaard, Development of an ELISA for vitellogenin in whole body homogenate of zebrafish (Danio rerio), Comp Biochem Physiol C Toxicol Pharmacol. 2001, 130(1):119-31