API

Event: 309

Key Event Title

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Reduction, Vitellogenin accumulation into oocytes and oocyte growth/development

Short name

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Reduction, Vitellogenin accumulation into oocytes and oocyte growth/development

Key Event Component

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Process Object Action
receptor-mediated endocytosis vitellogenins decreased
oocyte growth decreased
oocyte development decreased

Key Event Overview


AOPs Including This Key Event

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Stressors

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Level of Biological Organization

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Biological Organization
Cellular

Cell term

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Cell term
oocyte


Organ term

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Taxonomic Applicability

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Term Scientific Term Evidence Link
fathead minnow Pimephales promelas Moderate NCBI
Oryzias latipes Oryzias latipes Moderate NCBI

Life Stage Applicability

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Life stage Evidence
Adult, reproductively mature Strong

Sex Applicability

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Term Evidence
Female Strong

How This Key Event Works

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Vitellogenin from the blood is selectively taken up by competent oocytes via receptor-mediated endocytosis. Although vitellogenin receptors mediate the uptake, opening of intercellular channels through the follicular layers to the oocyte surface as the oocyte reaches a “critical” size is thought to be a key trigger in allowing vitellogenin uptake (Tyler and Sumpter 1996). Once critical size is achieved, concentrations in the plasma and temperature are thought to impose the primary limits on uptake (Tyler and Sumpter 1996). Uptake of vitellogenin into oocytes causes considerable oocyte growth during vitellogenesis, accounting for up to 95% of the final egg size in many fish (Tyler and Sumpter 1996). Given the central role of vitellogenesis in oocyte maturation, vitellogenin accumulation is a prominent feature used in histological staging of oocytes (e.g., (Leino et al. 2005; Wolf et al. 2004).


How It Is Measured or Detected

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Relative vitellogenin accumulation can be evaluated qualitatively using routine histological approaches (Leino et al. 2005; Wolf et al. 2004). Oocyte size can be evaluated qualitatively or quantitatively using routine histological and light microscopy and/or imaging approaches.


Evidence Supporting Taxonomic Applicability

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Oviparous vertebrates and invertebrates. Although hormonal regulation of vitellogenin synthesis and mechanisms of vitellogenin transport from the site of synthesis to the ovary vary between vertebrates and invertebrates (Wahli 1988), in both vertebrates and invertebrates, vitellogenin is incorporated into oocytes and cleaved to form yolk proteins.


References

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  • Leino R, Jensen K, Ankley G. 2005. Gonadal histology and characteristic histopathology associated with endocrine disruption in the adult fathead minnow. Environmental Toxicology and Pharmacology 19: 85-98.
  • Tyler C, Sumpter J. 1996. Oocyte growth and development in teleosts. Reviews in Fish Biology and Fisheries 6: 287-318.
  • Wolf JC, Dietrich DR, Friederich U, Caunter J, Brown AR. 2004. Qualitative and quantitative histomorphologic assessment of fathead minnow Pimephales promelas gonads as an endpoint for evaluating endocrine-active compounds: a pilot methodology study. Toxicol Pathol 32(5): 600-612.