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Key Event Title
Reduction, Plasma vitellogenin concentrations
Key Event Components
Key Event Overview
AOPs Including This Key Event
|AOP Name||Role of event in AOP||Point of Contact||Author Status||OECD Status|
|Aromatase inhibition leading to reproductive dysfunction||KeyEvent||Dan Villeneuve (send email)||Open for citation & comment||WPHA/WNT Endorsed|
|Androgen receptor agonism leading to reproductive dysfunction||KeyEvent||Dan Villeneuve (send email)||Open for citation & comment||WPHA/WNT Endorsed|
|Estrogen receptor antagonism leading to reproductive dysfunction||KeyEvent||Dan Villeneuve (send email)||Open for citation & comment||EAGMST Under Review|
|Prolyl hydroxylase inhibition||KeyEvent||Dalma Martinovic-Weigelt (send email)||Under Development: Contributions and Comments Welcome|
|Unknown MIE leading to reprodl||KeyEvent||Dalma Martinovic-Weigelt (send email)||Under Development: Contributions and Comments Welcome|
|TPO inhibition and impaired fertility||KeyEvent||June-Woo Park (send email)||Open for comment. Do not cite||Under Development|
|5α-reductase,female fish||KeyEvent||Young Jun Kim (send email)||Open for citation & comment||Under Development|
|AHR mediated epigenetic reproductive failure||KeyEvent||Jon Doering (send email)||Under development: Not open for comment. Do not cite|
|Adult, reproductively mature||High|
Key Event Description
Vitellogenin synthesized in the liver is secreted into the blood and circulates to the ovaries for uptake.
How It Is Measured or Detected
Vitellogenin concentrations in plasma are typically detected using enzyme linked Immunosorbent assay (ELISA; e.g., (Korte et al. 2000; Tyler et al. 1996; Holbech et al. 2001; Fenske et al. 2001). Although less specific and/or sensitive, determination of alkaline-labile phosphate or Western blotting has also been employed.
Domain of Applicability
Oviparous vertebrates synthesize yolk precursor proteins that are transported in the circulation for uptake by developing oocytes. Many invertebrates also synthesize vitellogenins that are taken up into developing oocytes via active transport mechanisms. However, invertebrate vitellogenins are transported in hemolymph or via other transport mechanisms rather than plasma.
- Fenske M, van Aerle R, Brack S, Tyler CR, Segner H. Development and validation of a homologous zebrafish (Danio rerio Hamilton-Buchanan) vitellogenin enzyme-linked immunosorbent assay (ELISA) and its application for studies on estrogenic chemicals. Comp Biochem Physiol C Toxicol Pharmacol. 2001. Jul;129(3):217-32.
- Holbech H, Andersen L, Petersen GI, Korsgaard B, Pedersen KL, Bjerregaard P. Development of an ELISA for vitellogenin in whole body homogenate of zebrafish (Danio rerio). Comp Biochem Physiol C Toxicol Pharmacol. 2001 Sep;130(1):119-31.
- Korte JJ, Kahl MD, Jensen KM, Mumtaz SP, Parks LG, LeBlanc GA, et al. 2000. Fathead minnow vitellogenin: complementary DNA sequence and messenger RNA and protein expression after 17B-estradiol treatment. Environmental Toxicology and Chemistry 19(4): 972-981.
- Tyler C, van der Eerden B, Jobling S, Panter G, Sumpter J. 1996. Measurement of vitellogenin, a biomarker for exposure to oestrogenic chemicals, in a wide variety of cyprinid fish. Journal of Comparative Physiology and Biology 166: 418-426.
- Wahli W. 1988. Evolution and expression of vitellogenin genes. Trends in Genetics. 4:227-232.