API

Event: 221

Key Event Title

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Reduction, Plasma vitellogenin concentrations

Short name

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Reduction, Plasma vitellogenin concentrations

Biological Context

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Level of Biological Organization
Organ


Organ term

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Organ term
blood plasma


Key Event Components

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Process Object Action
vitellogenins decreased

Key Event Overview


AOPs Including This Key Event

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Stressors

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Taxonomic Applicability

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Term Scientific Term Evidence Link
fathead minnow Pimephales promelas High NCBI
Oryzias latipes Oryzias latipes High NCBI
Danio rerio Danio rerio High NCBI

Life Stages

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Life stage Evidence
Adult, reproductively mature High

Sex Applicability

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Key Event Description

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Vitellogenin synthesized in the liver is secreted into the blood and circulates to the ovaries for uptake.


How It Is Measured or Detected

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Vitellogenin concentrations in plasma are typically detected using enzyme linked Immunosorbent assay (ELISA; e.g., (Korte et al. 2000; Tyler et al. 1996; Holbech et al. 2001; Fenske et al. 2001). Although less specific and/or sensitive, determination of alkaline-labile phosphate or Western blotting has also been employed.


Domain of Applicability

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Oviparous vertebrates synthesize yolk precursor proteins that are transported in the circulation for uptake by developing oocytes. Many invertebrates also synthesize vitellogenins that are taken up into developing oocytes via active transport mechanisms. However, invertebrate vitellogenins are transported in hemolymph or via other transport mechanisms rather than plasma.


References

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  • Fenske M, van Aerle R, Brack S, Tyler CR, Segner H. Development and validation of a homologous zebrafish (Danio rerio Hamilton-Buchanan) vitellogenin enzyme-linked immunosorbent assay (ELISA) and its application for studies on estrogenic chemicals. Comp Biochem Physiol C Toxicol Pharmacol. 2001. Jul;129(3):217-32.
  • Holbech H, Andersen L, Petersen GI, Korsgaard B, Pedersen KL, Bjerregaard P. Development of an ELISA for vitellogenin in whole body homogenate of zebrafish (Danio rerio). Comp Biochem Physiol C Toxicol Pharmacol. 2001 Sep;130(1):119-31.
  • Korte JJ, Kahl MD, Jensen KM, Mumtaz SP, Parks LG, LeBlanc GA, et al. 2000. Fathead minnow vitellogenin: complementary DNA sequence and messenger RNA and protein expression after 17B-estradiol treatment. Environmental Toxicology and Chemistry 19(4): 972-981.
  • Tyler C, van der Eerden B, Jobling S, Panter G, Sumpter J. 1996. Measurement of vitellogenin, a biomarker for exposure to oestrogenic chemicals, in a wide variety of cyprinid fish. Journal of Comparative Physiology and Biology 166: 418-426.
  • Wahli W. 1988. Evolution and expression of vitellogenin genes. Trends in Genetics. 4:227-232.